Hariharan, V and Chowdhury, AR and Rao S, S and Chakravortty, D and Basu, S (2023) phoP maintains the environmental persistence and virulence of pathogenic bacteria in mechanically stressed desiccated droplets. In: iScience, 26 (5).
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Abstract
Despite extensive studies on kinematic features of impacting drops, the effect of mechanical stress on desiccated bacteria-laden droplets remains unexplored. In the present study, we unveiled the consequences of the impaction of bacteria-laden droplets on solid surfaces and their subsequent desiccation on the virulence of an enteropathogen Salmonella typhimurium (STM). The methodology elucidated the deformation, cell-cell interactions, adhesion energy, and roughness in bacteria induced by impact velocity and low moisture because of evaporation. Salmonella retrieved from the dried droplets were used to understand fomite-mediated pathogenesis. The impact velocity-induced mechanical stress deteriorated the in vitro viability of Salmonella. Of interest, an uninterrupted bacterial proliferation was observed in macrophages at higher mechanical stress. Wild-type Salmonella under mechanical stress induced the expression of phoP whereas infecting macrophages. The inability of STM ΔphoP to grow in nutrient-rich dried droplets signifies the role of phoP in sensing the mechanical stress and maintaining the virulence of Salmonella.
| Item Type: | Journal Article |
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| Publication: | iScience |
| Publisher: | Elsevier Inc. |
| Additional Information: | The copyright of this article belongs to the Authors. |
| Keywords: | Applied physics; Biophysics; Microbiology |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology Division of Interdisciplinary Sciences > Interdisciplinary Centre for Energy Research Division of Mechanical Sciences > Mechanical Engineering |
| Date Deposited: | 14 Jun 2023 08:40 |
| Last Modified: | 14 Jun 2023 08:40 |
| URI: | https://eprints.iisc.ac.in/id/eprint/81875 |
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