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Cohesin dysfunction results in cell wall defects in budding yeast

Kothiwal, D and Gopinath, S and Laloraya, S (2021) Cohesin dysfunction results in cell wall defects in budding yeast. In: Genetics, 217 (1).

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Official URL: https://doi.org/10.1093/GENETICS/IYAA023


Cohesin is a conserved chromatin-binding multisubunit protein complex involved in diverse chromosomal transactions such as sisterchromatid cohesion, chromosome condensation, regulation of gene expression, DNA replication, and repair. While working with a budding yeast temperature-sensitive mutant, mcd1-1, defective in a cohesin subunit, we observed that it was resistant to zymolyase, indicating an altered cell wall organization. The budding yeast cell wall is a strong but elastic structure essential for maintenance of cell shape and protection from extreme environmental challenges. Here, we show that the cohesin complex plays an important role in cell wall maintenance. Cohesin mutants showed high chitin content in the cell wall and sensitivity to multiple cell wall stress-inducing agents. Interestingly, temperature-dependent lethality of cohesin mutants was osmoremedial, in a HOG1-MAPK pathway-dependent manner, suggesting that the temperature sensitivity of these mutants may arise partially from cell wall defects. Moreover, Mpk1 hyper-phosphorylation indicated activation of the cell wall integrity (CWI) signaling pathway in cohesin mutants. Genetic interaction analysis revealed that the CWI pathway is essential for survival of mcd1-1 upon additional cell wall stress. The cell wall defect was independent of the cohesion function and accompanied by misregulation of expression of several genes having cell wall-related functions. Our findings reveal a requirement of cohesin in maintenance of CWI that is independent of the CWI pathway, and that may arise from cohesin's role in regulating the expression of multiple genes encoding proteins involved in cell wall organization and biosynthesis. © 2020 The Author(s).

Item Type: Journal Article
Publication: Genetics
Publisher: Genetics Society of America
Additional Information: The copyright for this article belongs to the Authors.
Keywords: alkaline phosphatase; beta 1,3 glucan; chitin; chlorpromazine; clb2 protein; cohesin; complementary DNA; echinocandin; fks1 protein; galactose; gas1 protein; genomic DNA; hog1 protein; intercalating agent; mcd1 1 protein; mcd1 protein; mitogen activated protein kinase; mpk1 protein; nocodazole; restriction endonuclease; tunicamycin; unclassified drug; cell cycle protein; HOG1 protein, S cerevisiae; MCD1 protein, S cerevisiae; mitogen activated protein kinase; nonhistone protein; Saccharomyces cerevisiae protein; SLT2 protein, S cerevisiae, Article; biosynthesis; budding yeast; cell stress; cell survival; cell wall; controlled study; cytolysis; fluorescence activated cell sorting; gene expression; gene interaction; lethality; nonhuman; osmotic stress; priority journal; protein expression; protein phosphorylation; real time polymerase chain reaction; RNA sequencing; signal transduction; sister chromatid; temperature sensitivity; cell wall; genetics; MAPK signaling; metabolism; Saccharomyces cerevisiae, Cell Cycle Proteins; Cell Wall; Chromosomal Proteins, Non-Histone; MAP Kinase Signaling System; Mitogen-Activated Protein Kinases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 05 Jun 2023 06:53
Last Modified: 05 Jun 2023 06:53
URI: https://eprints.iisc.ac.in/id/eprint/81751

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