Pradhan, A and Mukkayyan, N and Jakkala, K and Ajitkumar, P (2021) Mycobacterial populations partly change the proportions of the cells undergoing asymmetric/symmetric divisions in response to glycerol levels in growth medium. In: Cells, 10 (5).
|
PDF
cell_10-5_2021.pdf - Published Version Download (3MB) | Preview |
Abstract
Twenty to thirty percent of the septating mycobacterial cells of the mid-log phase popula-tion showed highly deviated asymmetric constriction during division (ACD), while the remaining underwent symmetric constriction during division (SCD). The ACD produced short-sized cells (SCs) and normal/long-sized cells (NCs) as the sister–daughter cells, but with significant differential susceptibility to antibiotic/oxidative/nitrite stress. Here we report that, at 0.2% glycerol, formu-lated in the Middlebrook 7H9 medium, a significantly high proportion of the cells were divided by SCD. When the glycerol concentration decreased to 0.1% due to cell-growth/division, the ACD proportion gradually increased until the ACD:SCD ratio reached ~50:50. With further decrease in the glycerol levels, the SCD proportion increased with concomitant decrease in the ACD proportion. Maintenance of glycerol at 0.1%, through replenishment, held the ACD:SCD proportion at ~50:50. Transfer of the cells from one culture with a specific glycerol level to the supernatant from another culture, with a different glycerol level, made the cells change the ACD:SCD proportion to that of the culture from which the supernatant was taken. RT-qPCR data showed the possibility of diadenosine tetraphosphate phosphorylase (MSMEG_2932), phosphatidylinositol synthase (MSMEG_2933), and a Nudix family hydrolase (MSMEG_2936) involved in the ACD:SCD proportion-change in response to glycerol levels. We also discussed its physiological significance.
| Item Type: | Journal Article |
|---|---|
| Publication: | Cells |
| Publisher: | MDPI |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | antibiotic agent; biodiesel; complementary DNA; diadenosine tetraphosphate; FtsZ protein; glycerol; hydrolase; kanamycin; nitrite; Nudix hydrolase; phosphorylase; RNA 16S; sucrose; triacylglycerol; acid anhydride hydrolase; antioxidant; bis(5'-nucleosyl)tetraphosphatase (asymmetrical); cytidine diphosphate diacylglycerol inositol 3 phosphatidyltransferase; glycerol; inorganic pyrophosphatase; nudix hydrolases; RNA, acquired immune deficiency syndrome; Article; Bacillus subtilis; bacterium culture; carbon source; cell division; controlled study; DNA replication; electroporation; Escherichia coli; gene knockout; growth curve; microfluidics; morphotype; Mycobacterium; Mycobacterium smegmatis; Mycobacterium tuberculosis; Myxococcus xanthus; nonhuman; nucleotide metabolism; nutrient limitation; optical density; oxidative stress; physiological stress; quorum sensing; real time polymerase chain reaction; RNA extraction; supernatant; transfer cell; ultraviolet spectrophotometry; virus replication; cell proliferation; chemistry; culture medium; human; metabolism; microbiology; multidrug resistant tuberculosis; mutation; Mycobacterium bovis; tuberculosis, Acid Anhydride Hydrolases; Antioxidants; CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; Cell Proliferation; Culture Media; DNA, Complementary; Glycerol; Humans; Mutation; Mycobacterium bovis; Mycobacterium smegmatis; Mycobacterium tuberculosis; Oxidative Stress; Pyrophosphatases; RNA; Tuberculosis; Tuberculosis, Multidrug-Resistant |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 16 May 2023 09:43 |
| Last Modified: | 16 May 2023 09:43 |
| URI: | https://eprints.iisc.ac.in/id/eprint/81684 |
Actions (login required)
 |
View Item |
