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Genomic and phylogenetic analysis of a multidrug-resistant Burkholderia contaminans strain isolated from a patient with ocular infection

Lama, M and Chanakya, PP and Khamari, B and Peketi, ASK and Kumar, P and Nagaraja, V and Bulagonda, EP (2021) Genomic and phylogenetic analysis of a multidrug-resistant Burkholderia contaminans strain isolated from a patient with ocular infection. In: Journal of Global Antimicrobial Resistance, 25 . pp. 323-325.

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Official URL: https://doi.org/10.1016/j.jgar.2021.04.004


Objectives: The genus Burkholderia comprises rod-shaped, non-spore-forming, obligately aerobic Gram-negative bacteria that is found across diverse ecological niches. Burkholderia contaminans, an emerging pathogen associated with cystic fibrosis, is frequently isolated from contaminated medical devices in hospital settings. The aim of this study was to understand the genomic characteristics, antimicrobial resistance profile and virulence determinants of B. contaminans strain SBC01 isolated from the eye of a patient hit by a cow's tail. Methods: A hybrid sequence of isolate SBC01 was generated using Illumina HiSeq and Oxford Nanopore Technology platforms. Unicycler was used to assemble the hybrid genomic sequence. The draft genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline. Antimicrobial susceptibility testing was performed by VITEK®2. Antimicrobial resistance and virulence genes were identified using validated bioinformatics tools. Results: The assembled genome size is 8 841 722 bp with a G+C content of 66.33 distributed in 19 contigs. Strain SBC01 was found to possess several antimicrobial resistance and efflux pump genes. The isolate was susceptible to tetracyclines, meropenem and ceftazidime. Many genes encoding potential virulence factors were identified. Conclusion: Burkholderia contaminans SBC01 belonging to sequence type 482 (ST482) is a multidrug-resistant strain containing diverse antimicrobial resistance genes, revealing the risks associated with infections by new Burkholderia spp. The large G+C-rich genome has a myriad of virulence factors, highlighting its pathogenic potential. Thus, while providing insights into the antimicrobial resistance and virulence potential of this uncommon species, the present analysis will aid in understanding the evolution and speciation in the Burkholderia genus.

Item Type: Editorials/Short Communications
Publication: Journal of Global Antimicrobial Resistance
Publisher: Elsevier Ltd
Additional Information: The copyright for this article belongs to the Authors.
Keywords: aminoglycoside antibiotic agent; antibiotic agent; bacterial DNA; bacterial RNA; carbapenem derivative; ceftazidime; cephalosporin derivative; contig; genomic DNA; meropenem; monobactam derivative; quinoline derived antiinfective agent; ribosome RNA; tetracycline derivative; transfer RNA; untranslated RNA; virulence factor, agar gel electrophoresis; antibiotic resistance; antibiotic sensitivity; bacterial eye infection; bacterial genome; bacterial strain; bacterial virulence; bacterium isolation; bioinformatics; Burkholderia; Burkholderia contaminans; comparative study; controlled study; DNA base composition; female; genome analysis; genome size; human; microbial genome; multidrug resistance; multilocus sequence typing; nonhuman; Note; nucleotide sequence; phylogenetic tree; phylogeny; priority journal; animal; bovine; Burkholderia; eye infection; genomics; phylogeny, Animals; Burkholderia; Cattle; Eye Infections; Genome, Bacterial; Genomics; Humans; Phylogeny
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 16 May 2023 09:36
Last Modified: 16 May 2023 09:36
URI: https://eprints.iisc.ac.in/id/eprint/81675

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