Singh, A and Babu, K and Pandey, P (2021) Ethanol-induced sedative behavior: An assay to investigate increased dopamine signaling in caenorhabditis elegans. In: Bio-protocol, 11 (13).
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Abstract
Dopamine (DA) signaling affects locomotion, feeding, learning, and memory in C. elegans. Various assays have been developed to study the proteins involved in these behaviors; however, these assays show behavioral output only when there is a drastic change in DA levels. We designed an assay capable of observing behavioral output even with only slight alterations in DA levels. To achieve this, we designed a behavioral paradigm where we combined C. elegans movement with ethanol (EtOH) administration. The behavioral response to alcohol/EtOH and susceptibility to alcohol-use disorders (AUDs) have been linked to DA. Our assay correlates an increase in DA levels due to EtOH and movement obstruction due to a dry surface to a circular sedative behavior, which we designated as EtOH-induced sedative (EIS) behavior.
| Item Type: | Journal Article |
|---|---|
| Publication: | Bio-protocol |
| Publisher: | Bio-protocol LLC |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | C. elegans; Dopamine (DA); EtOH (Ethanol); Sedative behavior |
| Department/Centre: | Division of Biological Sciences > Centre for Neuroscience |
| Date Deposited: | 16 May 2023 09:17 |
| Last Modified: | 16 May 2023 09:17 |
| URI: | https://eprints.iisc.ac.in/id/eprint/81662 |
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