Labeling approaches for DNA-PAINT super-resolution imaging

Banerjee, A and Anand, M and Ganji, M (2023) Labeling approaches for DNA-PAINT super-resolution imaging. In: Nanoscale, 15 (14). pp. 6563-6580.

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Abstract

Super-resolution imaging is becoming a commonly employed tool to visualize biological targets in unprecedented detail. DNA-PAINT is one of the single-molecule localization microscopy-based super-resolution imaging modalities allowing the ultra-high-resolution imaging with superior multiplexing capabilities. We discuss the importance of patterned DNA nanostructures in demonstrating the capabilities of DNA-PAINT and the design of various combinations of imager-docking strand pairs for imaging. Central to the implementation of DNA-PAINT imaging in a biological context is the generation of docking strand-conjugated binders against the target molecules. Several researchers have developed a variety of labelling probes for improving resolution while also providing multiplexing capabilities for the broader application of DNA-PAINT. This review provides a comprehensive summary of the repertoire of labelling probes used for DNA-PAINT in cells and the strategies implemented to chemically modify them with a docking strand.

Item Type:	Journal Article
Publication:	Nanoscale
Publisher:	NLM (Medline)
Additional Information:	The copyright for this article belongs to Royal Society of Chemistry.
Keywords:	DNA; nanomaterial, chemistry; fluorescence microscopy; procedures; single molecule imaging, DNA; Microscopy, Fluorescence; Nanostructures; Single Molecule Imaging
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	28 May 2023 05:57
Last Modified:	28 May 2023 05:57
URI:	https://eprints.iisc.ac.in/id/eprint/81496

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