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Red light-activable biotinylated copper(II) complex-functionalized gold nanocomposite (Biotin-Cu@AuNP) towards targeted photodynamic therapy

Musib, D and Upadhyay, A and Pal, M and Raza, MK and Saha, I and Kunwar, A and Roy, M (2023) Red light-activable biotinylated copper(II) complex-functionalized gold nanocomposite (Biotin-Cu@AuNP) towards targeted photodynamic therapy. In: Journal of Inorganic Biochemistry, 243 .

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Official URL: https://doi.org/10.1016/j.jinorgbio.2023.112183


We report the synthesis and characterization of red-light activable gold nanoparticle functionalized with biotinylated copper(II) complex of general molecular formula, [Cu(L3)(L6)]-AuNPs (Biotin-Cu@AuNP), where L3 = N-(3-((E)-3,5-di-tert-butyl-2-hydroxybenzylideneamino)-4-hydroxyphenyl)-5-((3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, L6 = 5-(1,2-dithiolan-3-yl)-N-(1,10-phenanthrolin-5-yl)pentanamide, which was explored for their photophysical, theoretical and photo-cytotoxic potentials. The nanoconjugate exhibits differential uptake in biotin positive and biotin negative cancer cells as well as normal cells. The nanoconjugate also shows remarkable photodynamic activity against biotin positive A549 (IC50: 13 μg/mL in red light; >150 μg/mL in dark) and HaCaT (IC50: 23 μg/mL in red light; >150 μg/mL in dark) cells under red light (600–720 nm, 30 Jcm−2) irradiation, with significantly high photo-indices (PI>15). The nanoconjugate is less toxic to HEK293T (biotin negative) and HPL1D (normal) cells. Confocal microscopy confirms preferential mitochondrial and partly cytoplasmic localization of Biotin-Cu@AuNP in A549 cells. Several photo-physical and theoretical studies reveal the red light-assisted generation of singlet oxygen (1O2) (Ф (1O2) =0.68) as a reactive oxygen species (ROS) which results in remarkable oxidative stress and mitochondrial membrane damage, leading to caspase 3/7-dependent apoptosis of A549 cells. Overall, the nanocomposite (Biotin-Cu@AuNP) exhibiting red light-assisted targeted photodynamic activity has emerged as the ideal next generation PDT agents. © 2023 Elsevier Inc.

Item Type: Journal Article
Publication: Journal of Inorganic Biochemistry
Publisher: Elsevier Inc.
Additional Information: The copyright for this article belongs to Elsevier Inc.
Keywords: Biotinylated copper(II)-functionalized gold nanocomposite; Caspase 3/7-dependent apoptosis; Mitochodrial membrane disruption; Singlet oxygen (1O2); Targeted PDT in red-light
Department/Centre: Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited: 02 May 2023 09:14
Last Modified: 02 May 2023 09:14
URI: https://eprints.iisc.ac.in/id/eprint/81240

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