Agent-Based Model of Heterogeneous T-Cell Activation in Vitro

Majumdar, S and Molina-París, C and Nandi, D and Lythe, G (2021) Agent-Based Model of Heterogeneous T-Cell Activation in Vitro. [Book Chapter]

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Abstract

We combine modelling and in vitro measurement of T-cell properties at the level of individual cells. Rather than seek to model kinetics using one differential equation per cell population, our computational model describes the dynamics of a cohort of cells in terms of a series of discrete events, one after the other, occurring to one cell at a time. Two types of heterogeneity are explicitly simulated: cell surface marker expression that is time-dependent and varies from cell to cell and spatial heterogeneity that arises from local influences such as the proximity of IL-2-producing cells. Neither storing 50,000 individual cell instances and their attributes nor following the kinetics over times of order 48 h and producing simulated flow cytometry plots, is too taxing for a modern desktop computer. In vitro, the kinetics of the activation of cohorts of CD4 T cells from BALB/c mice was studied, under stimulus with soluble or plate-bound anti-CD3 or a combination of PMA and ionomycin. © Springer Nature Switzerland AG 2021. All rights reserved.

Item Type:	Book Chapter
Publication:	Mathematical, Computational and Experimental T Cell Immunology
Publisher:	Springer International Publishing
Additional Information:	The copyright for this article belongs to Springer International Publishing.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	17 Mar 2023 10:00
Last Modified:	30 Mar 2023 09:06
URI:	https://eprints.iisc.ac.in/id/eprint/81039

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