Rho-dependent transcription termination is the dominant mechanism in Mycobacterium tuberculosis

Ahmad, E and Mitra, A and Ahmed, W and Mahapatra, V and Hegde, SR and Sala, C and Cole, ST and Nagaraja, V (2023) Rho-dependent transcription termination is the dominant mechanism in Mycobacterium tuberculosis. In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1866 (2).

PDF bio_bio_act_1866-2_2023.pdf - Published Version Restricted to Registered users only Download (7MB) | Request a copy

Abstract

Intrinsic and Rho-dependent transcription termination mechanisms regulate gene expression and recycle RNA polymerase in bacteria. Both the modes are well studied in Escherichia coli, and a few other organisms. The understanding of Rho function is limited in most other bacteria including mycobacteria. Here, we highlight the dominance of Rho-dependent termination in mycobacteria and validate Rho as a key regulatory factor. The lower abundance of intrinsic terminators, high cellular levels of Rho, and its genome-wide association with a majority of transcriptionally active genes indicate the pronounced role of Rho-mediated termination in Mycobacterium tuberculosis (Mtb). Rho modulates the termination of RNA synthesis for both protein-coding and stable RNA genes in Mtb. Concordantly, the depletion of Rho in mycobacteria impact its growth and enhances the transcription read-through at 3′ ends of the transcription units. We demonstrate that MtbRho is catalytically active in the presence of RNA with varied secondary structures. These properties suggest an evolutionary adaptation of Rho as the efficient and preponderant mode of transcription termination in mycobacteria. © 2023 Elsevier B.V.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to Elsevier B.V.
Keywords:	article; catalysis; evolutionary adaptation; genetic transcription; genome-wide association study; Mycobacterium; Mycobacterium tuberculosis; nonhuman; protein secondary structure; regulatory mechanism; RNA gene; RNA synthesis; transcription initiation; transcription termination
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	16 Mar 2023 06:12
Last Modified:	16 Mar 2023 06:12
URI:	https://eprints.iisc.ac.in/id/eprint/80965

Actions (login required)

View Item