Structural and related studies on Mevo lectin from Methanococcus voltae A3: The first thorough characterization of an archeal lectin and its interactions

Sivaji, N and Suguna, K and Surolia, A and Vijayan, M (2021) Structural and related studies on Mevo lectin from Methanococcus voltae A3: The first thorough characterization of an archeal lectin and its interactions. In: Glycobiology, 31 (3). pp. 315-328.

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Abstract

Crystallographic and solution studies of Mevo lectin and its complexes, the first effort of its kind on an archeal lectin, reveal a structure similar to β-prism I fold lectins from plant and animal sources, but with a quaternary association involving a ring structure with seven-fold symmetry. Each subunit in the heptamer carries one sugar binding site on the first Greek key motif. The oligomeric interface is primarily made up of a parallel β-sheet involving a strand of Greek key I of one subunit and Greek key' from a neighboring subunit. The crystal structures of the complexes of the lectin with mannose, αMan(1,2)αMan, αMan(1,3)αMan, a mannotriose and a mannopentose revealed a primary binding site similar to that found in other mannose specific β-prism I fold lectins. The complex with αMan(1,3)αMan provides an interesting case in which a few subunits have the reducing end at the primary binding site, while the majority have the nonreducing end at the primary binding site. The structures of complexes involving the trisaccharide and the pentasaccharide exhibit cross-linking among heptameric molecules. The observed arrangements may be relevant to the multivalency of the lectin. Phylogenetic analysis of amino acid sequences indicates that Mevo lectin is closer to β-prism I fold animal lectins than with those of plant origin. The results presented here reinforce the conclusion regarding the existence of lectins in all three domains of life. It would also appear that lectins evolved to the present form before the three domains diverged. © 2020 The Author(s) 2020. Published by Oxford University Press. All rights reserved.

Item Type:	Journal Article
Publication:	Glycobiology
Publisher:	Oxford University Press
Additional Information:	The copyright for this article belongs to Oxford University Press.
Keywords:	lectin; mannose; mevo lectin; pentasaccharide; trisaccharide; unclassified drug; lectin; oligosaccharide, amino acid sequence; Article; binding site; controlled study; crystal structure; gel filtration; gene duplication; hydrogen bond; isothermal titration calorimetry; Methanococcus voltae; molecular cloning; molecular weight; nonhuman; photon correlation spectroscopy; phylogeny; priority journal; protein carbohydrate interaction; protein expression; protein purification; protein quaternary structure; sequence alignment; size exclusion chromatography; chemistry; conformation; Methanococcus; molecular model; X ray crystallography, Amino Acid Sequence; Crystallography, X-Ray; Lectins; Methanococcus; Models, Molecular; Molecular Conformation; Oligosaccharides
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	08 Mar 2023 12:49
Last Modified:	08 Mar 2023 12:49
URI:	https://eprints.iisc.ac.in/id/eprint/80855

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