A Cerium Vanadate Nanozyme with Specific Superoxide Dismutase Activity Regulates Mitochondrial Function and ATP Synthesis in Neuronal Cells

Singh, N and NaveenKumar, SK and Geethika, M and Mugesh, G (2021) A Cerium Vanadate Nanozyme with Specific Superoxide Dismutase Activity Regulates Mitochondrial Function and ATP Synthesis in Neuronal Cells. In: Angewandte Chemie - International Edition, 60 (6). pp. 3121-3130.

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Abstract

Nanoparticles that functionally mimic the activity of metal-containing enzymes (metallo-nanozymes) are of therapeutic importance for treating various diseases. However, it is still not clear whether such nanozymes can completely substitute the function of natural enzymes in living cells. In this work, we show for the first time that a cerium vanadate (CeVO4) nanozyme can substitute the function of superoxide dismutase 1 and 2 (SOD1 and SOD2) in the neuronal cells even when the natural enzyme is down-regulated by specific gene silencing. The nanozyme prevents the mitochondrial damage in SOD1- and SOD2-depleted cells by regulating the superoxide levels and restores the physiological levels of the anti-apoptotic Bcl-2 family proteins. Furthermore, the nanozyme effectively prevents the mitochondrial depolarization, leading to a significant improvement in the cellular levels of ATP under oxidative stress

Item Type:	Journal Article
Publication:	Angewandte Chemie - International Edition
Publisher:	Wiley-VCH Verlag
Additional Information:	The copyright for this article belongs to Wiley-VCH Verlag.
Keywords:	Cerium compounds; Cytology; Metal nanoparticles; Mitochondria; Vanadium compounds, Bcl-2 family proteins; Cellular levels; Cerium vanadate; Mitochondrial damage; Mitochondrial function; Physiological levels; Super oxide dismutase; Superoxide dismutase activities, Enzymes, adenosine triphosphate; biomimetic material; cerium; copper zinc superoxide dismutase; manganese superoxide dismutase; nanomaterial; small interfering RNA; superoxide; superoxide dismutase; vanadic acid, chemistry; cytology; genetics; human; metabolism; mitochondrion; nerve cell; RNA interference; tumor cell line, Adenosine Triphosphate; Biomimetic Materials; Cell Line, Tumor; Cerium; Humans; Mitochondria; Nanostructures; Neurons; RNA Interference; RNA, Small Interfering; Superoxide Dismutase; Superoxide Dismutase-1; Superoxides; Vanadates
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	15 Feb 2023 10:11
Last Modified:	15 Feb 2023 10:11
URI:	https://eprints.iisc.ac.in/id/eprint/80363

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