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MRNA Recruiting eIF4 Factors Involved in Protein Synthesis and Its Regulation

Mishra, RK and Datey, A and Hussain, T (2020) MRNA Recruiting eIF4 Factors Involved in Protein Synthesis and Its Regulation. In: Biochemistry, 59 (1). pp. 34-46.

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Official URL: https://doi.org/10.1021/acs.biochem.9b00788


The recruitment of mRNA onto the ribosome affects not only global translation but also the spatial and temporal fine-tuning of eukaryotic translation initiation. The eukaryotic initiation factor 4 (eIF4) factors, namely, eIF4G, eIF4E, eIF4A, and eIF4B, facilitate the recruitment of mRNA onto the preassembled 43S pre-initiation complex (PIC), thus leading to the formation of the 48S PIC. Several biochemical and genetic studies have established the roles of eIF4 factors; however, the available structural information is limited. While structures of some of the individual components and subcomplexes are available, the structural details of activated mRNA bound to eIF4 factors (eIF4-mRNA) are missing. Structural characterization of the eIF4-mRNA in association with the 43S PIC in different organisms is crucial for a detailed understanding of mRNA recruitment and for exploiting the structural differences for possible drug design.

Item Type: Journal Article
Publication: Biochemistry
Publisher: American Chemical Society
Additional Information: The copyright for this article belongs to American Chemical Society.
Keywords: Biochemistry; Chemistry, Eukaryotic initiation factor; Eukaryotic translation; Individual components; Preinitiation complex; Structural characterization; Structural details; Structural differences; Structural information, Biosynthesis, cap binding protein; helicase; initiation factor 4A; initiation factor 4B; initiation factor 4E; initiation factor 4G; messenger RNA; initiation factor 4F; messenger RNA; protein binding, gene function; gene interaction; genetic trait; human; priority journal; protein assembly; protein function; protein structure; protein synthesis; Review; ribosome; RNA translation; translation initiation; animal; chemistry; metabolism; protein domain; Saccharomyces cerevisiae, Animals; Eukaryotic Initiation Factor-4F; Humans; Protein Binding; Protein Domains; RNA, Messenger; Saccharomyces cerevisiae
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 06 Feb 2023 09:58
Last Modified: 06 Feb 2023 09:58
URI: https://eprints.iisc.ac.in/id/eprint/79933

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