Comparative evaluation of structure and characteristic of peptidyl-prolyl cis-trans isomerase proteins and their function in Salmonella Typhimurium stress responses and virulence

Kumawat, M and Singh, PK and Rananaware, SR and Ahlawat, S (2020) Comparative evaluation of structure and characteristic of peptidyl-prolyl cis-trans isomerase proteins and their function in Salmonella Typhimurium stress responses and virulence. In: Folia Microbiologica, 65 (1). pp. 161-171.

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Abstract

Peptidyl-prolyl cis-trans isomerases (PPIase) exhibit chaperone activity and assist in protein folding by increasing the rate of cis-trans transition on proline-peptide bonds. The current study aimed to identify and characterize three genes, ppiA, ppiB, and ppiC, which encode proteins of the PPIase family in the bacterium Salmonella enterica serovar Typhimurium. Salmonella Typhimurium is a facultative intracellular zoonotic pathogen that causes food- and water-borne gastroenteritis in humans (leading to bacteremia in immune-compromised subjects). Recombinant clones for the three genes were constructed and sequenced and the sequences submitted to NCBI GenBank. Three-dimensional structures for the corresponding proteins were predicted by comparative modeling. A maximum-likelihood phylogenetic gene tree constructed for the three genes showed a low evolutionary mean diversity, indicating strong evolutionary conservation. Further, single-gene deletion mutant strains, generated for the respective genes, were observed to be more susceptible to the stationary phase of growth and heat stress conditions and showed reduced survival within macrophage cells line. The present study thus indicates that ppiA, ppiB, and ppiC genes are conserved among Salmonella genome, are critical for the growth of Salmonella Typhimurium in the examined stress conditions, and may play a role in its responses and virulence

Item Type:	Journal Article
Publication:	Folia Microbiologica
Publisher:	Springer
Additional Information:	The copyright for this article belongs to the Springer.
Keywords:	bacterial protein; peptidylprolyl isomerase, animal; animal salmonellosis; bacterial genome; chemistry; chicken; comparative study; enzymology; genetics; microbiology; pathogenicity; phylogeny; physiological stress; Salmonella enterica serovar Typhimurium; virulence, Animals; Bacterial Proteins; Chickens; Genome, Bacterial; Peptidylprolyl Isomerase; Phylogeny; Salmonella Infections, Animal; Salmonella typhimurium; Stress, Physiological; Virulence
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	02 Feb 2023 09:37
Last Modified:	02 Feb 2023 09:37
URI:	https://eprints.iisc.ac.in/id/eprint/79758

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