Glycodelin regulates the numbers and function of peripheral natural killer cells

Dixit, A and Karande, AA (2020) Glycodelin regulates the numbers and function of peripheral natural killer cells. In: Journal of Reproductive Immunology, 137 .

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Abstract

Natural killer (NK) cells comprise of ∼70% of the immune cell population in the maternal decidua and ∼15% of the mononuclear cells in the peripheral blood. The decidual NK cells capable of producing high levels of cytokines are functionally distinct from the peripheral NK cells that exhibit high cytotoxicity. The numbers of peripheral NK cells and their cytotoxicity potential have been correlated with pregnancy outcome. In the same context, glycodelin, an immunomodulatory protein, has been recognized to be essential for the establishment and maintenance of pregnancy, and its’ reduced levels are associated with recurrent spontaneous abortions. We investigated the effect of glycodelin on the peripheral NK cells. Our results reveal that glycodelin suppresses the cytotoxicity of peripheral NK cells via downregulating perforin, granzyme B and IFNγ. Glycodelin also induces caspase-dependent death in only activated peripheral NK cells, the effect suggested to be mediated by glycodelin upon engaging with the CD7 cell surface receptor. Thus, during pregnancy, glycodelin modulates the function and the number of cytotoxic NK cells that pose a deleterious effect on the fetus, a semi-allograft. This study provides insights into the mechanism of the regulatory effect of glycodelin on NK cells and could possibly be exploited for the management of miscarriages

Item Type:	Journal Article
Publication:	Journal of Reproductive Immunology
Publisher:	Elsevier Ireland Ltd
Additional Information:	The copyright for this article belongs to the Elsevier Ireland Ltd
Keywords:	CD7 antigen; gamma interferon; granzyme B; perforin; placenta protein 14; CD7 antigen; gamma interferon; granzyme; GZMB protein, human; IFNG protein, human; PAEP protein, human; perforin; placenta protein 14, apoptosis; Article; controlled study; cytokine release; cytotoxicity; cytotrophoblast; female; fetus wastage; flow cytometry; human; human cell; immunocompetent cell; immunomodulation; interferon production; Jurkat cell line; lymphocyte activation; natural killer cell; natural killer T cell; peripheral blood mononuclear cell; pregnancy; pregnancy outcome; priority journal; recurrent abortion; spontaneous abortion; T lymphocyte; cell communication; down regulation; immunology; lymphocyte count; metabolism; mononuclear cell; natural killer cell; nidation; primary cell culture; recurrent abortion; trophoblast; tumor cell line, Abortion, Habitual; Antigens, CD7; Cell Communication; Cell Line, Tumor; Down-Regulation; Embryo Implantation; Female; Glycodelin; Granzymes; Humans; Interferon-gamma; Killer Cells, Natural; Leukocytes, Mononuclear; Lymphocyte Count; Natural Killer T-Cells; Perforin; Primary Cell Culture; Trophoblasts
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	02 Feb 2023 09:33
Last Modified:	02 Feb 2023 09:33
URI:	https://eprints.iisc.ac.in/id/eprint/79753

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