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The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans

Kolathur, KK and Sharma, P and Kadam, NY and Shahi, N and Nishitha, A and Babu, K and Mishra, SK (2022) The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans. In: FEBS Letters .

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Official URL: https://doi.org/10.1002/1873-3468.14555


The ubiquitin-like protein Hub1/UBL-5 associates with proteins non-covalently. Hub1 promotes alternative splicing and splicing of precursor mRNAs with weak introns in yeast and mammalian cells; however, its splicing function has remained elusive in multicellular organisms. Here, we demonstrate the splicing function of Hub1/UBL-5 in the free-living nematode Caenorhabditis elegans. Hub1/UBL-5 binds to the HIND-containing splicing factors Snu66/SART-1 and PRP-38 and associates with other spliceosomal proteins. C. elegans hub1/ubl-5 mutants die at the Larval 3 stage and show splicing defects for selected targets, similar to the mutants in yeast and mammalian cells. UBL-5 complemented growth and splicing defects in Schizosaccharomyces pombe hub1 mutants, confirming its functional conservation. Thus, UBL-5 is important for C. elegans development and plays a conserved pre-mRNA splicing function. © 2022 Federation of European Biochemical Societies.

Item Type: Journal Article
Publication: FEBS Letters
Publisher: John Wiley and Sons Inc
Additional Information: The copyright for this article belongs to John Wiley and Sons Inc
Keywords: outron-containing transcripts; pre-mRNA splicing; spliceosome; trans-splicing and C. elegans
Department/Centre: Division of Biological Sciences > Centre for Neuroscience
Date Deposited: 31 Jan 2023 06:46
Last Modified: 31 Jan 2023 06:46
URI: https://eprints.iisc.ac.in/id/eprint/79605

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