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A Versatile Suspended Lipid Membrane System for Probing Membrane Remodeling and Disruption

Sannigrahi, A and Rai, VH and Chalil, MV and Chakraborty, D and Meher, SK and Roy, R (2022) A Versatile Suspended Lipid Membrane System for Probing Membrane Remodeling and Disruption. In: Membranes, 12 (12).

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Official URL: https://doi.org/10.3390/membranes12121190


Artificial membrane systems can serve as models to investigate molecular mechanisms of different cellular processes, including transport, pore formation, and viral fusion. However, the current, such as SUVs, GUVs, and the supported lipid bilayers suffer from issues, namely high curvature, heterogeneity, and surface artefacts, respectively. Freestanding membranes provide a facile solution to these issues, but current systems developed by various groups use silicon or aluminum oxide wafers for fabrication that involves access to a dedicated nanolithography facility and high cost while conferring poor membrane stability. Here, we report the development, characterization and applications of an easy-to-fabricate suspended lipid bilayer (SULB) membrane platform leveraging commercial track-etched porous filters (PCTE) with defined microwell size. Our SULB system offers a platform to study the lipid composition-dependent structural and functional properties of membranes with exceptional stability. With dye entrapped in PCTE microwells by SULB, we show that sphingomyelin significantly augments the activity of pore-forming toxin, Cytolysin A (ClyA) and the pore formation induces lipid exchange between the bilayer leaflets. Further, we demonstrate high efficiency and rapid kinetics of membrane fusion by dengue virus in our SULB platform. Our suspended bilayer membrane mimetic offers a novel platform to investigate a large class of biomembrane interactions and processes. © 2022 by the authors.

Item Type: Journal Article
Publication: Membranes
Publisher: MDPI
Additional Information: The copyright for this article belongs to the Authors.
Keywords: Alumina; Aluminum oxide; Cell membranes; Phospholipids; Pore size; Silicon wafers; System stability; Viruses, Bi-layer; Cytolysin A; Lipid membranes; Membrane disruption; Membrane system; Micro wells; Pore formation; Pore forming toxins; Suspended bilayer; Virus fusion, Lipid bilayers
Department/Centre: Division of Mechanical Sciences > Chemical Engineering
Date Deposited: 27 Jan 2023 09:37
Last Modified: 27 Jan 2023 09:37
URI: https://eprints.iisc.ac.in/id/eprint/79557

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