Haque, OI and Chandrasekaran, A and Nabi, F and Ahmad, O and Marques, JP and Ahmad, T (2022) A novel compound heterozygous BEST1 gene mutation in two siblings causing autosomal recessive bestrophinopathy. In: BMC Ophthalmology, 22 (1).
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Abstract
Purpose: To describe the clinical features, imaging characteristics, and genetic test results associated with a novel compound heterozygous mutation of the BEST1 gene in two siblings with autosomal recessive bestrophinopathy. Methods: Two siblings underwent a complete ophthalmic examination, including dilated fundus examination, fundus photography, fundus autofluorescence imaging, spectral-domain optical coherence tomography, fluorescein angiography, electroretinography, and electrooculography. A clinical diagnosis of autosomal recessive bestrophinopathy was established based on ocular examination and multimodal retinal imaging. Subsequently, clinical exome sequencing consisting of a panel of 6670 genes was carried out to confirm the diagnosis and assess genetic alterations in the protein-coding region of the genome of the patients. The identified mutations were tested in the two affected siblings and one of their parents. Results: Two siblings (a 17-year-old female and a 15-year-old male) presented with reduced visual acuity and bilaterally symmetrical subretinal deposits of hyperautofluorescent materials in the posterior pole, which showed staining in the late phase of fluorescein angiogram. Spectral-domain optical coherence tomography demonstrated hyperreflective subretinal deposits and subretinal fluid accumulation. Both patients shared two mutations in the protein-coding region of the BEST1 gene, c.103G > A, p.(Glu35Lys) and c.313C > A, p.(Arg105Ser) (a novel disease-causing mutation). Sanger sequencing confirmed that the unaffected mother of the proband was carrying p.(Glu35Lys) variant in a heterozygous state. Conclusions: We have identified and described the phenotype of a novel disease-causing mutation NM004183.4:c.313C > A, p.(Arg105Ser) in a heterozygous state along with a previously reported mutation NM004183.4:c.103G > A, p.(Glu35Lys) of the BEST1 gene in two related patients with autosomal recessive bestrophinopathy. © 2022, The Author(s).
| Item Type: | Journal Article |
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| Publication: | BMC Ophthalmology |
| Publisher: | BioMed Central Ltd |
| Additional Information: | The copyright for this article belongs to BioMed Central. |
| Keywords: | Autosomal recessive bestrophinopathy; BEST1; Bestrophin-1; Genetics; Inherited retinal dystrophy |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 25 Jan 2023 06:12 |
| Last Modified: | 25 Jan 2023 06:12 |
| URI: | https://eprints.iisc.ac.in/id/eprint/79489 |
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