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Salmonella typhimurium encoded cold shock protein e is essential for motility and biofilm formation

Ray, S and Da Costa, R and Thakur, S and Nandi, D (2020) Salmonella typhimurium encoded cold shock protein e is essential for motility and biofilm formation. In: Microbiology (United Kingdom), 166 (5). pp. 460-473.

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Official URL: https://doi.org/10.1099/mic.0.000900


The ability of bacteria to form biofilms increases their survival under adverse environmental conditions. Biofilms have enormous medical and environmental impact; consequently, the factors that influence biofilm formation are an important area of study. In this investigation, the roles of two cold shock proteins (CSP) during biofilm formation were investigated in Salmonella Typhimurium, which is a major foodborne pathogen. Among all CSP transcripts studied, the expression of cspE (STM14_0732) was higher during biofilm growth. The cspE deletion strain (ΔcspE) did not form biofilms on a cholesterol coated glass surface; however, complementation with WT cspE, but not the F30V mutant, was able to rescue this phenotype. Transcript levels of other CSPs demonstrated up-regulation of cspA (STM14_4399) in ΔcspE. The cspA deletion strain (ΔcspA) did not affect biofilm formation; however, ΔcspEΔcspA exhibited higher biofilm formation compared to ΔcspE. Most likely, the higher cspA amounts in ΔcspE reduced biofilm formation, which was corroborated using cspA over-expression studies. Further functional studies revealed that ΔcspE and ΔcspEΔcspA exhibited slow swimming but no swarming motility. Although cspA over-expression did not affect motility, cspE complementation restored the swarming motility of ΔcspE. The transcript levels of the major genes involved in motility in ΔcspE demonstrated lower expression of the class III (fliC, motA, cheY), but not class I (flhD) or class II (fliA, fliL), flagellar regulon genes. Overall, this study has identified the interplay of two CSPs in regulating two biological processes: CspE is essential for motility in a CspA-independent manner whereas biofilm formation is CspA-dependent. © 2020 The Authors.

Item Type: Journal Article
Publication: Microbiology (United Kingdom)
Publisher: Microbiology Society
Additional Information: The copyright for this article belongs to the Authors.
Keywords: cholesterol; cold shock protein; cold shock protein e; unclassified drug; bacterial protein; cold shock protein, Article; atomic force microscopy; bacterial growth; bacterial strain; biofilm; controlled study; extracellular fluid; gene amplification; gene deletion; gene expression; nonhuman; priority journal; real time polymerase chain reaction; Salmonella enterica serovar Typhimurium; scanning electron microscopy; surface property; biofilm; cellular, subcellular and molecular biological phenomena and functions; gene expression regulation; genetic complementation; genetics; growth, development and aging; movement (physiology); mutation; physiology; Salmonella enterica serovar Typhimurium; ultrastructure; upregulation, Bacterial Proteins; Biofilms; Biological Phenomena; Cold Shock Proteins and Peptides; Gene Expression Regulation, Bacterial; Genetic Complementation Test; Movement; Mutation; Salmonella typhimurium; Up-Regulation
Department/Centre: Division of Biological Sciences > Biochemistry
UG Programme
Date Deposited: 24 Jan 2023 04:45
Last Modified: 24 Jan 2023 04:45
URI: https://eprints.iisc.ac.in/id/eprint/79302

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