Zein-Alginate-Phosphatidylcholine Nanocomplex Efficiently Delivers Lycopene and Lutein over Dietary-Derived Carotenoid Mixed Micelles in Caco-2 Cells

Shwetha, HJ and Arathi, BP and Beto Mukherjee, M and Ambedkar, R and Shivaprasad, S and Raichur, AM and Lakshminarayana, R (2022) Zein-Alginate-Phosphatidylcholine Nanocomplex Efficiently Delivers Lycopene and Lutein over Dietary-Derived Carotenoid Mixed Micelles in Caco-2 Cells. In: Journal of Agricultural and Food Chemistry, 70 (49). pp. 15474-15486.

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Abstract

This study evaluated the potency of zein-alginate-phosphatidylcholine nanoparticles (NPs) on bioaccessibility/intestinal uptake of encapsulated lycopene (LY) and lutein (LT) versus dietary absorption using simulated digestion and human intestinal Caco-2 cells. LY-zein-alginate-PC (LYZAP) and LT-zein-alginate-PC (LTZAP) NPs yield desired properties, which exhibit sustained release and are suitable for oral administration. Interestingly, co-treatment of LYZAP + LTZAP showed better release of carotenoids instead of individual treatment at intestinal pH. Bioaccessibility, cellular uptake, and basolateral secretion of LY and LT from NPs were significantly enhanced than micellar carotenoids (dietary mode of absorption). The increased absorption of carotenoids from NPs correlated with triglyceride levels. The intestinal cell uptake of carotenoids by nanoencapsulation may be due to endocytosis, paracellular, and SRB-1 protein-mediated transport. Overall, LYZAP and LTZAP NPs possess superior properties to control the release and cellular uptake of unique or distinct carotenoids. The inclusion of alginate and phosphatidylcholine in zein-based nanoencapsulation could be a promising strategy to improve carotenoid bioavailability. © 2022 American Chemical Society.

Item Type:	Journal Article
Publication:	Journal of Agricultural and Food Chemistry
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to American Chemical Society.
Keywords:	Biochemistry; Cells; Cytology; Micelles; Molecular biology; Nanoparticles, Bioaccessibility; Caco-2 cell uptake of nanoparticle; Caco-2 cells; Carotenoid-zein-alginate-phosphatidylcholine nanoparticle; Cell uptake; Cellular uptake; Lycopenes; Phosphatidyl choline; Property; SRB-1-mediated transport, Carotenoids
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	14 Jan 2023 06:53
Last Modified:	14 Jan 2023 06:53
URI:	https://eprints.iisc.ac.in/id/eprint/79150

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