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The utility of nuclear magnetic resonance spectroscopy in assisted reproduction: NMR in Assisted Reproduction

Asampille, G and Cheredath, A and Joseph, D and Adiga, SK and Atreya, HS (2020) The utility of nuclear magnetic resonance spectroscopy in assisted reproduction: NMR in Assisted Reproduction. In: Open Biology, 10 (11).

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Official URL: https://doi.org/10.1098/rsob.200092


Infertility affects approximately 15-20 of individuals of reproductive age worldwide. Over the last 40 years, assisted reproductive technology (ART) has helped millions of childless couples. However, ART is limited by a low success rate and risk of multiple gestations. Devising methods for selecting the best gamete or embryo that increases the ART success rate and prevention of multiple gestation has become one of the key goals in ART today. Special emphasis has been placed on the development of non-invasive approaches, which do not require perturbing the embryonic cells, as the current morphology-based embryo selection approach has shortcomings in predicting the implantation potential of embryos. An observed association between embryo metabolism and viability has prompted researchers to develop metabolomics-based biomarkers. Nuclear magnetic resonance (NMR) spectroscopy provides a non-invasive approach for the metabolic profiling of tissues, gametes and embryos, with the key advantage of having a minimal sample preparation procedure. Using NMR spectroscopy, biologically important molecules can be identified and quantified in intact cells, extracts or secretomes. This, in turn, helps to map out the active metabolic pathways in a system. The present review covers the contribution of NMR spectroscopy in assisted reproduction at various stages of the process. © 2020 The Authors.

Item Type: Journal Article
Publication: Open Biology
Publisher: Royal Society Publishing
Additional Information: The copyright for this article belongs to The Authors.
Keywords: biological marker, antibody specificity; embryo culture; female; human; infertility therapy; male; metabolomics; nuclear magnetic resonance spectroscopy; procedures; systems biology, Biomarkers; Embryo Culture Techniques; Female; Humans; Magnetic Resonance Spectroscopy; Male; Metabolomics; Organ Specificity; Reproductive Techniques, Assisted; Systems Biology
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Division of Chemical Sciences > Sophisticated Instruments Facility (Continued as NMR Research Centre)
Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited: 11 Jan 2023 04:42
Last Modified: 11 Jan 2023 04:42
URI: https://eprints.iisc.ac.in/id/eprint/79030

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