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Genomic islands and the evolution of livestock-associated Staphylococcus aureus genomes

Rao, RT and Sharma, S and Sivakumar, N and Jayakumar, K (2020) Genomic islands and the evolution of livestock-associated Staphylococcus aureus genomes. In: Bioscience Reports, 40 (11).

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Official URL: https://doi.org/10.1042/BSR20202287


Background: Genomic Islands (GIs) are commonly believed to be relics of horizontal transfer and associated with specific metabolic capacities, including virulence of the strain. Horizontal gene transfer (HGT) plays a vital role in the acquisition of GIs and the evolution and adaptation of bacterial genomes. Objective: The present study was designed to predict the GIs and role of HGT in evolution of livestock-associated Staphylococcus aureus (LA-SA). Methods: GIs were predicted with two methods namely, Ensemble algorithm for Genomic Island Detection (EGID) tool, and Seq word Sniffer script. Functional characterization of GI elements was performed with clustering of orthologs. The putative donor predictions of GIs was done with the aid of the pre GI database. Results: The present study predicted a pan of 46 GIs across the LA-SA genomes. Functional characterization of GI sequences revealed few unique results like the presence of metabolic operons like leuABCD and folPK genes in GIs and showed the importance of GIs in the adaptation to the host niche. The developed framework for GI donor prediction results revealed Rickettsia and Mycoplasma as the major donors of GI elements. Conclusions: The role of GIs during the evolutionary race of LA-SA could be concluded from the present study. Niche adaptation of LA-SA enhanced presumably due to these GIs. Future studies could focus on the evolutionary relationships between Rickettsia and Mycoplasma sp. with S. aureus and also the evolution of Leucine/Isoleucine mosaic operon (leuABCD). © 2020 Portland Press Ltd. All rights reserved.

Item Type: Journal Article
Publication: Bioscience Reports
Publisher: Portland Press Ltd
Additional Information: The copyright for this article belongs to the Author(S).
Keywords: isoleucine; leucine, Article; bacterial gene; bacterial genome; bioinformatics; comparative genomics; controlled study; evolutionary adaptation; folPK gene; gene sequence; genetic algorithm; genetic database; genomic island; horizontal gene transfer; leuABCD gene; livestock; Mycoplasma; nonhuman; operon; prediction; Rickettsia; Staphylococcus aureus; animal; biology; comparative study; gene expression regulation; genetics; horizontal gene transfer; isolation and purification; livestock; microbiology; molecular evolution; pathogenicity; Staphylococcus aureus; Staphylococcus infection; veterinary medicine; virulence, Animals; Computational Biology; Databases, Genetic; Evolution, Molecular; Gene Expression Regulation, Bacterial; Gene Transfer, Horizontal; Genome, Bacterial; Genomic Islands; Livestock; Staphylococcal Infections; Staphylococcus aureus; Virulence
Department/Centre: Division of Biological Sciences > Central Animal Facility (Formerly Primate Research Laboratory)
Date Deposited: 10 Jan 2023 06:14
Last Modified: 10 Jan 2023 06:14
URI: https://eprints.iisc.ac.in/id/eprint/78998

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