Fabrication of Microfluidic Impedance Cytometer for Leukocytes Analysis

Subramaniam, S and Ahmed, U and Benerjee, S and Jain, P and Chowdhury, P (2019) Fabrication of Microfluidic Impedance Cytometer for Leukocytes Analysis. In: 11th International Conference on Communication Systems and Networks, COMSNETS 2019, 7 January 2019 - 11 January 2019, Bengaluru, pp. 829-832.

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Abstract

In this paper, we demonstrate a very simple and cost effective method to fabricate three dimensional top and bottom electrodes for the application of leukocytes analysis using impedance based cytometry. The developed microfluidic impedance cytometry has compactness and no requirement of optical interface. The off-chip sample preparation was carried out to analyze the leukocytes cell differential count. Human blood was treated with saponin/formic acid to lyse erythrocytes and subsequently quench was added to halt the lysis of leukocytes. A detailed fabrication process of microelectrodes and microfluidic chip are presented in this paper. The Microfluidic Impedance Cytometry (MIC) consists of a microfluidic chip with micro patterned electrodes that measure the impedance of each cell at two frequencies and sub-population of leukocyte count was performed after recording the data from lock-in amplifier.

Item Type:	Conference Poster
Publication:	2019 11th International Conference on Communication Systems and Networks, COMSNETS 2019
Publisher:	Institute of Electrical and Electronics Engineers Inc.
Additional Information:	The copyright for this article belongs to Institute of Electrical and Electronics Engineers Inc.
Keywords:	Blood; Cost effectiveness; Fabrication; Fluidic devices; Microelectrodes; Micromachining; Population statistics, Bottom electrodes; Cost-effective methods; Fabrication process; Lock-in amplifier; Micro-patterned electrodes; Microfluidic chip; Optical interfaces; Sample preparation, Microfluidics
Department/Centre:	Autonomous Societies / Centres > Society for Innovation and Development
Date Deposited:	27 Dec 2022 07:08
Last Modified:	27 Dec 2022 07:08
URI:	https://eprints.iisc.ac.in/id/eprint/78583

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