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Bioremediation of Pb(II) ions from aqueous solution using extracellular polymeric substances (EPS) purified from Pseudomonas fluorescens

Vimalnath, S and Kumar, RV and Carsten, S and Subramanian, S (2019) Bioremediation of Pb(II) ions from aqueous solution using extracellular polymeric substances (EPS) purified from Pseudomonas fluorescens. In: 29th International Mineral Processing Congress, IMPC 2018, 17 - 21 September 2018, Moscow, pp. 3170-3179.

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The effect of EPS components namely protein, polysaccharide and biosurfactant purified from Pseudomonas fluorescens was investigated for the bioremediation of Pb(II) ions in aqueous solution. Two parameters, namely the amount of EPS component and the concentration of Pb(II) ions were optimized for maximum Pb(II) ions uptake. The EPS components interacted with Pb(II) ions were characterized using energy dispersive X-ray analysis (EDAX) to ascertain the presence of Pb and by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) to reveal the functional groups involved in Pb(II) binding. The maximum Pb(II) binding capacities of protein, polysaccharide and biosurfactant were found to be 11.4, 0.55 and 0.11 mg mg -1 respectively. The biosorption processes followed the Langmuir model. The Gibbs free energy (∆G 0 ) values of -23.6, -20.9 and -23.2 kJ mol -1 for Pb(II) biosorption by protein, polysaccharide and biosurfactant respectively, attested to spontaneous, chemisorption processes. The ATR-FTIR results revealed the presence and involvement of amino, hydroxyl, carboxyl, phosphate, and methyl functional groups in Pb(II) binding and EDAX analysis confirmed the interaction of Pb with EPS components. The Pb(II) binding mechanisms of EPS have been discussed. The results demonstrate the potential of the purified EPS components of P. fluorescens for removal of Pb(II) ions from aqueous solution.

Item Type: Conference Paper
Publication: IMPC 2018 - 29th International Mineral Processing Congress
Publisher: Canadian Institute of Mining, Metallurgy and Petroleum
Additional Information: The copyright for this article belongs to Canadian Institute of Mining, Metallurgy and Petroleum.
Keywords: Bacteria; Binding energy; Biomolecules; Bioremediation; Biosorption; Biotechnology; Energy dispersive X ray analysis; Fourier transform infrared spectroscopy; Free energy; Gibbs free energy; Ions; Polysaccharides; Proteins; Purification; Supersaturation; Surface active agents; X ray diffraction analysis, Attenuated total reflection Fourier transform infrared spectroscopy; Binding capacities; Binding mechanisms; Bio surfactant; Biosorption process; Energy dispersive Xray analyses (EDAX); Extracellular polymeric substances; Pseudomonas fluorescens, Lead compounds
Department/Centre: Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited: 28 Nov 2022 07:01
Last Modified: 28 Nov 2022 07:01
URI: https://eprints.iisc.ac.in/id/eprint/78025

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