Simultaneous sensing of ferritin and apoferritin proteins using an iron-responsive dye and evaluation of physiological parameters associated with serum iron estimation

Dey, N and Ali, A and Kamra, M and Bhattacharya, S (2019) Simultaneous sensing of ferritin and apoferritin proteins using an iron-responsive dye and evaluation of physiological parameters associated with serum iron estimation. In: Journal of Materials Chemistry B, 7 (6). pp. 986-993.

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Abstract

An iron-responsive optical probe has been developed for simultaneous sensing of both ferritin and apoferritin proteins at pH 7.4 in water. The compound showed an exclusive response (turn-off signal) towards ferritin among a wide range of proteins even at nanomolar concentration. In contrast, apoferritin dissociates the preformed iron complex and revives the green colored fluorescence of the native probe (turn-on signal). Subsequently, various parameters associated with the serum iron level are evaluated, which are beneficial for clinical diagnosis of many iron-related diseases, including anemia. Estimation of iron was achieved in a wide range of edible plant materials as well as pharmaceutical formulations. Subsequently, different kinds of natural water samples were screened for quantification of soluble iron contents. In addition to traditional spectroscopic tools, dye-coated paper strips were developed as an alternative strategy for onsite 'instrument-free' detection of iron. Highly specific bioimaging of Fe 3+ was achieved in cervical cancer cells (HeLa).

Item Type:	Journal Article
Publication:	Journal of Materials Chemistry B
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright for this article belongs to the Royal Society of Chemistry.
Keywords:	Body fluids; Diagnosis; Physiological models; Probes; Proteins, Cervical cancer cells; Clinical diagnosis; Nanomolar concentration; Natural water samples; Pharmaceutical formulation; Physiological parameters; Simultaneous sensing; Spectroscopic tool, Iron compounds, Diagnosis; Estimation; Iron Compounds; Measuring Instruments; Paper Strips; Parameters; Proteins; Water, apoferritin; ferritin; fluorescent dye; fresh water; iron, blood; chemistry; confocal microscopy; HeLa cell line; human; pH; quantum theory; spectrophotometry; ultraviolet radiation, Apoferritins; Ferritins; Fluorescent Dyes; Fresh Water; HeLa Cells; Humans; Hydrogen-Ion Concentration; Iron; Microscopy, Confocal; Quantum Theory; Spectrophotometry; Ultraviolet Rays
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	18 Nov 2022 09:36
Last Modified:	18 Nov 2022 09:36
URI:	https://eprints.iisc.ac.in/id/eprint/77995

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