Subban, K and Subramani, R and Madambakkam Srinivasan, VP and Johnpaul, M and Chelliah, J (2019) Salicylic acid as an effective elicitor for improved taxol production in endophytic fungus Pestalotiopsis microspora. In: PLoS ONE, 14 (2).
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Abstract
Salicylic acid (SA) is an effective elicitor to increase taxol production in Pestalotiopsis microspora. Addition of SA at the concentration of 300 μM yielded taxol 625.47 μg L -1 , 45- fold higher than that of the control. Elicitation of the role of SA in the fungal taxol biosynthetic pathway revealed that SA enhanced reactive oxygen species and lipid peroxidation of unsaturated fatty acids of P. microspora mycelia. This oxidative process stimulates isoprene biosynthetic pathway by triggering expression of the geranylgeranyl pyrophosphate synthase gene leading to improved biosynthesis of taxol in P. microspora.
| Item Type: | Journal Article |
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| Publication: | PLoS ONE |
| Publisher: | Public Library of Science |
| Additional Information: | The copyright for this article belongs to the Author(s). |
| Keywords: | catalase; farnesyl trans transferase; glutathione; hydroxymethylglutaryl coenzyme A reductase; isoprene; lipid peroxidase; paclitaxel; peroxidase; reactive oxygen metabolite; salicylic acid; superoxide dismutase; unsaturated fatty acid; farnesyl trans transferase; fungal protein; paclitaxel; salicylic acid, antioxidant activity; Article; biosynthesis; controlled study; endophytic fungus; fungal biomass; gene expression; geranylgeranyl pyrophosphate synthase gene; lipid peroxidation; mycelium; nonhuman; Pestalotiopsis microspora; protein content; protein expression; Ascomycetes; drug effect; gene expression regulation; metabolism, Ascomycota; Farnesyltranstransferase; Fungal Proteins; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Paclitaxel; Salicylic Acid |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 28 Oct 2022 06:00 |
| Last Modified: | 28 Oct 2022 06:00 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77727 |
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