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Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming

Kollaran, AM and Joge, S and Kotian, HS and Badal, D and Prakash, D and Mishra, A and Varma, M and Singh, V (2019) Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming. In: iScience, 13 . pp. 305-317.

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Official URL: https://doi.org/10.1016/j.isci.2019.02.028


Pathogenic Organism; Biological Sciences; Microbiology © 2019 The Authors Swarming in Pseudomonas aeruginosa is a coordinated movement of bacteria over semisolid surfaces (0.5%–0.7% agar). On soft agar, P. aeruginosa exhibits a dendritic swarm pattern, with multiple levels of branching. However, the swarm patterns typically vary depending upon the experimental design. In the present study, we show that the pattern characteristics of P. aeruginosa swarm are highly environment dependent. We define several quantifiable, macroscale features of the swarm to study the plasticity of the swarm, observed across different nutrient formulations. Furthermore, through a targeted screen of 113 two-component system (TCS)loci of the P. aeruginosa strain PA14, we show that forty-four TCS genes regulate swarming in PA14 in a contextual fashion. However, only four TCS genes—fleR, fleS, gacS, and PA14_59770—were found essential for swarming. Notably, many swarming-defective TCS mutants were found highly efficient in biofilm formation, indicating opposing roles for many TCS loci.

Item Type: Journal Article
Publication: iScience
Publisher: Elsevier Inc.
Additional Information: The copyright for this article belongs to the Authors.
Keywords: Biological Sciences; Microbiology; Pathogenic Organism
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Division of Interdisciplinary Sciences > Robert Bosch Centre for Cyber Physical Systems
Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited: 28 Oct 2022 07:52
Last Modified: 28 Oct 2022 07:52
URI: https://eprints.iisc.ac.in/id/eprint/77699

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