Temporally Controlled Multienzyme Catalysis Using a Dissipative Supramolecular Nanozyme

Solra, M and Das, S and Srivastava, A and Sen, B and Rana, S (2022) Temporally Controlled Multienzyme Catalysis Using a Dissipative Supramolecular Nanozyme. In: ACS Applied Materials and Interfaces .

PDF ACS_app_mat_int_14-40_45096-45109_2022.pdf - Published Version Restricted to Registered users only Download (7MB) | Request a copy

Abstract

The development of superior functional enzyme mimics (nanozymes) is essential for practical applications, including point-of-care diagnostics, biotechnological applications, biofuels, and environmental remediation. Nanozymes with the ability to control their catalytic activity in response to external fuels offer functionally valuable platforms mimicking nonequilibrium systems in nature. Herein, we fabricated a supramolecular coordination bonding-based dynamic vesicle that exhibits multienzymatic activity. The supramolecular nanozyme shows effective laccase-like catalytic activity with a KM value better than the native enzyme and higher stability in harsh conditions. Besides, the nanostructure demonstrates an efficient peroxidase-like activity with NADH peroxidase-like properties. Generation of luminescence from luminol and oxidation of dopamine are efficiently catalyzed by the nanozyme with high sensitivity, which is useful for point-of-care detections. Notably, the active nanozyme exhibits dynamic laccase-mimetic activity in response to pH variation, which has never been explored before. While a neutral/high pH leads to the self-assembly, a low pH disintegrates the assembled nanostructures and consequently turns off the nanozyme activity. Altogether, the self-assembled Cu2+-based vesicular nanostructure presents a pH-fueled dissipative system demonstrating effective temporally controlled multienzymatic activity.

Item Type:	Journal Article
Publication:	ACS Applied Materials and Interfaces
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to American Chemical Society.
Keywords:	biocatalysis; dissipative assembly; multienzyme mimic; nanozyme; supramolecular
Department/Centre:	Division of Chemical Sciences > Materials Research Centre
Date Deposited:	28 Oct 2022 07:50
Last Modified:	28 Oct 2022 07:50
URI:	https://eprints.iisc.ac.in/id/eprint/77679

Actions (login required)

View Item