Structural and functional implications of leucine-rich repeats in toll-like receptor1 subfamily

Dey, D and Dhar, D and Das, S and Maulik, A and Basu, S (2022) Structural and functional implications of leucine-rich repeats in toll-like receptor1 subfamily. In: Journal of Biosciences, 47 (4).

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Abstract

Leucine-rich repeats (LRRs) – the protein–protein and protein–ligand interaction motif of proteins participating in a plethora of functions in plants, vertebrates, invertebrates, and prokaryotes – are a fascinating piece of conserved yet versatile structural motif. In toll-like receptors (TLRs), this domain forms the extracellular part that is preceded by an intracellular toll/interleukin-1 receptor (TIR) domain. The extracellular part is crucial for recognizing a structurally diverse set of viral, bacterial, fungal, and parasite-derived components, while the TIR domain is recruited for activation of downstream signaling following recognition. The distinct ability of the paralogs TLR1 and TLR6 to dimerize with TLR2 and recognize different ligands intrigued and motivated us to exchange the dimerizing and ligand-binding residues between TLR1/6 and note the effect on dimer formation and ligand binding. The appreciable sequence modification brought about no significant alteration in the native scaffold of the motif, as revealed from the comparison of simulations with wild-type dimers. Moreover, docking of the exchanged ligands to the variant proteins supported favorable binding. Thus, the structural stability and the functional plasticity offered by the motif might be the reason for its extensive use across cellular functions and life forms, a feature crucial for coevolution and the knowledge essential for therapeutics.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Springer
Additional Information:	The copyright for this article belongs to the Springer.
Keywords:	Chimera; leucine-rich repeats; molecular dynamics simulation; structural alignment; toll-like receptors
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	27 Oct 2022 09:16
Last Modified:	27 Oct 2022 09:16
URI:	https://eprints.iisc.ac.in/id/eprint/77621

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