Anantha Krishna, TH and Kamalraj, S and Anikisetty, M and Naidu, KA and Surin, WR and Jayabaskaran, C (2019) Inhibition of thrombin, an unexplored function of retinoic acid. In: Biochemistry and Biophysics Reports, 18 .
|
PDF
bio_bio_phy_rep_18_2019.pdf - Published Version Download (801kB) | Preview |
Abstract
Retinoic acid, a derivative of vitamin A, is known to possess in vivo anti-inflammatory, anti-platelet and fibrinolytic activities. We have investigated the in vitro thrombin and platelet aggregation inhibitory activities of vitamin A (retinol) and its derivatives, retinoic acid and retinaldehyde. The thrombin enzymatic assay was performed fluorimetrically to assess the inhibition of thrombin (Sigma and plasma). Retinoic acid, retinaldehyde and retinol exhibited potent inhibition of thrombin, with IC50 values of 67μg/ml, 74μg/ml and 152μg/ml, respectively for the inhibition of thrombin (Sigma); and 49μg/ml, 74μg/ml and 178μg/ml, respectively for the inhibition of thrombin (plasma). Amongst vitamin A and its derivatives, retinoic acid showed the highest inhibition of both the forms of thrombin. Vitamin A and its derivatives also displayed remarkable inhibition of platelet aggregation. This is the first report of vitamin A and its derivatives showing inhibition of thrombin and platelet aggregation in vitro.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry and Biophysics Reports |
| Publisher: | Elsevier B.V. |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | heparin; retinal; retinoic acid; retinol; thrombin; ticlopidine, animal cell; animal experiment; antiplatelet activity; Article; blood clotting; blood sampling; concentration response; controlled study; enzyme activity; enzyme inhibition; fluorometry; IC50; in vitro study; male; nonhuman; rat; thrombocyte aggregation |
| Department/Centre: | Division of Biological Sciences > Biochemistry Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 23 Oct 2022 06:53 |
| Last Modified: | 23 Oct 2022 06:53 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77508 |
Actions (login required)
 |
View Item |
