Nanguneri, S and Pramod, RT and Efimova, N and Das, D and Jose, M and Svitkina, T and Nair, D (2019) Characterization of nanoscale organization of f-actin in morphologically distinct dendritic spines in vitro using supervised learning. In: eNeuro, 6 (4).
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Abstract
The cytoarchitecture of a neuron is very important in defining morphology and ultrastructure. Although there is a wealth of information on the molecular components that make and regulate these ultrastructures, there is a dearth of understanding of how these changes occur or how they affect neurons in health and disease. Recent advances in nanoscale imaging which resolve cellular structures at the scale of tens of nanometers below the limit of diffraction enable us to understand these structures in fine detail. However, automated analysis of these images is still in its infancy. Towards this goal, attempts have been made to automate the detection and analysis of the cytoskeletal organization of microtubules. To date, evaluation of the nanoscale organization of filamentous actin (F-actin) in neuronal compartments remains challenging. Here, we present an objective paradigm for analysis which adopts supervised learning of nanoscale images of F-actin network in excitatory synapses, obtained by single molecule based super-resolution light microscopy. We have used the proposed analysis to understand the heterogeneity in the organization of F-actin in dendritic spines of primary neuronal cultures from rodents. Our results were validated using ultrastructural data obtained from platinum replica electron microscopy (PREM). The automated analysis approach was used to differentiate the heterogeneity in the nanoscale organization of F-actin in primary neuronal cultures from wild-type (WT) and a transgenic mouse model of Alzheimer’s disease (APPSwe/PS1∆E9).
| Item Type: | Journal Article |
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| Publication: | eNeuro |
| Publisher: | Society for Neuroscience |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | F actin; actin, Alzheimer disease; animal cell; Article; controlled study; dendritic spine; electron microscopy; female; in vitro study; learning algorithm; male; microscopy; mouse; nanoimaging; nerve cell culture; nonhuman; primary cell culture; protein analysis; protein localization; single molecule imaging; supervised machine learning; synapse; ultrastructure; animal; dendritic spine; hippocampus; image processing; procedures; Sprague Dawley rat; ultrastructure, Actins; Animals; Dendritic Spines; Female; Hippocampus; Image Processing, Computer-Assisted; In Vitro Techniques; Male; Microscopy; Rats, Sprague-Dawley; Supervised Machine Learning |
| Department/Centre: | Division of Biological Sciences > Centre for Neuroscience |
| Date Deposited: | 23 Oct 2022 06:49 |
| Last Modified: | 23 Oct 2022 06:49 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77506 |
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