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Physics of self-assembly and morpho-topological changes of Klebsiella pneumoniae in desiccating sessile droplets

Rasheed, A and Hegde, O and Chatterjee, R and Sampathirao, SR and Chakravortty, D and Basu, S (2023) Physics of self-assembly and morpho-topological changes of Klebsiella pneumoniae in desiccating sessile droplets. In: Journal of Colloid and Interface Science, 629 . pp. 620-631.

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Official URL: https://doi.org/10.1016/j.jcis.2022.09.100


Hypothesis: The bacteria suspended in pure water self-assemble into unique patterns depending on bacteria-bacteria, bacteria-substrate and bacteria-liquid interactions. The physical forces acting on bacteria vary based on their respective spatial location inside the droplet cause an assorted magnitude of physical stress. The shear and dehydration induced stress on pathogens(bacteria) in drying bio-fluid droplets alters the viability and infectivity. Experiments: We have investigated the flow and desiccation-driven self-assembly of Klebsiella pneumoniae in the naturally evaporating sessile droplets. Klebsiella pneumoniae exhibits extensive changes in its morphology and forms unique patterns as the droplet dries, revealing hitherto unexplored rich physics governing its survival and infection strategies. Self-assembly of bacteria at the droplet contact line is characterized by order-to-disorder packing transitions with high packing densities and excessive deformations (analysed using scanning electron microscopy and atomic force microscopy). In contrast, thin-film instability-led hole formation at the center of the droplet engenders spatial packing of bacteria analogous to honeycomb weathering. Findings: Self-assembly favors the bacteria at the rim of the droplet, leading to enhanced viability and pathogenesis on the famously known “coffee ring” of the droplet compared to the bacteria present at the center of the droplet residue. Mechanistic insights gained via our study can have far-reaching implications for bacterial infection through droplets, e.g., through open wounds.

Item Type: Journal Article
Publication: Journal of Colloid and Interface Science
Publisher: Academic Press Inc.
Additional Information: The copyright for this article belongs to the Authors.
Keywords: Driers (materials); Drops; Morphology; Scanning electron microscopy; Self assembly; Sols, And infectivity; Bio-colloids; Drying droplets; Klebsiella pneumoniae; Liquid interactions; Pure water; Self-assemble; Sessile droplet; Topological changes; Viability, Bacteria
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Division of Mechanical Sciences > Mechanical Engineering
Date Deposited: 19 Oct 2022 08:34
Last Modified: 19 Oct 2022 08:34
URI: https://eprints.iisc.ac.in/id/eprint/77425

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