Damke, PP and Di Guilmi, AM and Fernández Varela, P and Velours, C and Marsin, S and Veaute, X and Machouri, M and Gunjal, GV and Rao, DN and Charbonnier, J-B and Radicella, JP (2019) Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori. In: Nature Communications, 10 (1).
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Abstract
Horizontal gene transfer through natural transformation is a major driver of antibiotic resistance spreading in many pathogenic bacterial species. In the case of Gram-negative bacteria, and in particular of Helicobacter pylori, the mechanisms underlying the handling of the incoming DNA within the periplasm are poorly understood. Here we identify the protein ComH as the periplasmic receptor for the transforming DNA during natural transformation in H. pylori. ComH is a DNA-binding protein required for the import of DNA into the periplasm. Its C-terminal domain displays strong affinity for double-stranded DNA and is sufficient for the accumulation of DNA in the periplasm, but not for DNA internalisation into the cytoplasm. The N-terminal region of the protein allows the interaction of ComH with a periplasmic domain of the inner-membrane channel ComEC, which is known to mediate the translocation of DNA into the cytoplasm. Our results indicate that ComH is involved in the import of DNA into the periplasm and its delivery to the inner membrane translocator ComEC.
| Item Type: | Journal Article |
|---|---|
| Publication: | Nature Communications |
| Publisher: | Nature Research |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | bacterial protein; cell DNA; ComH protein; double stranded DNA; extrachromosomal DNA; periplasmic protein; unclassified drug; bacterial DNA; bacterial protein; cell surface receptor; DNA; DNA receptor, antibiotic resistance; bacterium; DNA; gene transfer; identification method; protein, animal cell; animal experiment; Article; bacterial cell wall; bacterial translocation; bacterium transformation; controlled study; cytoplasm; DNA sequence; duplex polymerase chain reaction; ectopic expression; electroporation; fluorescence microscopy; gel mobility shift assay; gene sequence; Helicobacter pylori; horizontal gene transfer; nonhuman; protein analysis; protein expression; protein transport; sedimentation rate; surface plasmon resonance; ultracentrifugation; genetics; Helicobacter pylori; horizontal gene transfer; metabolism; periplasm; transport at the cellular level, Bacteria (microorganisms); Helicobacter pylori; Negibacteria, Bacterial Proteins; Biological Transport; DNA; DNA, Bacterial; Gene Transfer, Horizontal; Helicobacter pylori; Periplasm; Receptors, Cell Surface; Transformation, Bacterial |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 12 Oct 2022 09:10 |
| Last Modified: | 12 Oct 2022 09:10 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77397 |
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