Dahiya, Y and Rose, S and Thapliyal, S and Bhardwaj, S and Prasad, M and Babu, K (2019) Differential regulation of innate and learned behavior by Creb1/Crh-1 in caenorhabditis elegans. In: Journal of Neuroscience, 39 (40). pp. 7934-7946.
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Abstract
Memory formation is crucial for the survival of animals. Here, we study the effect of different crh-1 Caenorhabditis elegans homolog of mammalian cAMP response element binding protein 1 (CREB1) isoforms on the ability of C. elegans to form long-term memory (LTM). Null mutants in creb1/crh-1 are defective in LTM formation across phyla. We show that a specific isoform of CREB1/CRH-1, CRH-1e, is primarily responsible for memory related functions of the transcription factor in C. elegans. Silencing of CRH-1e-expressing neurons during training for LTM formation abolishes the LTM of the animal. Further, CRH-1e expression in RIM neurons is sufficient to rescue LTM defects of creb1/crh-1-null mutants. We go on to show that apart from being LTM defective, creb1/crh-1-null animals show defects in innate chemotaxis behavior. We further characterize the amino acids K247 and K266 as responsible for the LTM related functions of CREB1/CRH-1 while being dispensable for its innate chemotaxis behavior. These findings provide insight into the spatial and temporal workings of a crucial transcription factor that can be further exploited to find CREB1 targets involved in the process of memory formation. Copyright © 2019 Dahiya et al.
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal of Neuroscience |
| Publisher: | Society for Neuroscience |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | cyclic AMP responsive element binding protein; lysine; Caenorhabditis elegans protein; CRH-1 protein, C elegans; transcription factor, animal cell; Article; associative learning; Caenorhabditis elegans; chemotaxis; controlled study; CRISPR based mutagenesis; gene expression; gene silencing; learning; long term memory; mutagenesis; mutant; nonhuman; priority journal; amino acid substitution; animal; Caenorhabditis elegans; chemotaxis; drive; genetics; learning; long term memory; memory; metabolism; nerve cell; nerve cell network; optogenetics; physiology; sensation; transgenic animal, Amino Acid Substitution; Animals; Animals, Genetically Modified; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Chemotaxis; Instinct; Learning; Memory; Memory, Long-Term; Nerve Net; Neurons; Optogenetics; Sensation; Transcription Factors |
| Department/Centre: | Division of Biological Sciences > Centre for Neuroscience |
| Date Deposited: | 13 Oct 2022 06:06 |
| Last Modified: | 13 Oct 2022 06:06 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77343 |
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