Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc

Nellas, I and Iyer, KV and Iglesias-Artola, JM and Pippel, M and Nadler, A and Eaton, S and Dye, NA (2022) Hedgehog signaling can enhance glycolytic ATP production in the Drosophila wing disc. In: EMBO Reports .

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Abstract

Adenosine triphosphate (ATP) production and utilization is critically important for animal development. How these processes are regulated in space and time during tissue growth remains largely unclear. We used a FRET-based sensor to dynamically monitor ATP levels across a growing tissue, using the Drosophila larval wing disc. Although steady-state levels of ATP are spatially uniform across the wing pouch, inhibiting oxidative phosphorylation reveals spatial differences in metabolic behavior, whereby signaling centers at compartment boundaries produce more ATP from glycolysis than the rest of the tissue. Genetic perturbations indicate that the conserved Hedgehog signaling pathway can enhance ATP production by glycolysis. Collectively, our work suggests the existence of a homeostatic feedback loop between Hh signaling and glycolysis, advancing our understanding of the connection between conserved developmental patterning genes and ATP production during animal tissue development.

Item Type:	Journal Article
Publication:	EMBO Reports
Publisher:	John Wiley and Sons Inc
Additional Information:	The copyright for this article belongs to John Wiley and Sons Inc.
Keywords:	adenosine triphosphate; Drosophila; glycolysis; Hedgehog; metabolism
Department/Centre:	Division of Mechanical Sciences > Mechanical Engineering
Date Deposited:	12 Oct 2022 08:54
Last Modified:	12 Oct 2022 08:54
URI:	https://eprints.iisc.ac.in/id/eprint/77307

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