Gupta, A and Rangarajan, PN (2022) Coordinate regulation of methanol utilization pathway genes of Komagataella phaffii by transcription factors and chromatin modifiers. In: Frontiers in Microbiology, 13 .
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Abstract
The methylotrophic yeast Komagataella phaffii (a.k.a. Pichia pastoris) harbors a methanol utilization (MUT) pathway, enabling it to utilize methanol as the sole source of carbon. The nexus between transcription factors such as Mxr1p and Trm1p and chromatin-modifying enzymes in the regulation of genes of MUT pathway has not been well studied in K. phaffii. Using transcriptomics, we demonstrate that Gcn5, a histone acetyltransferase, and Gal83, one of the beta subunits of nuclear-localized SNF1 (sucrose non-fermenting 1) kinase complex are essential for the transcriptional regulation by the zinc finger transcription factors Mxr1p and Trm1p. We conclude that interactions among Gcn5, Snf1, Mxr1p, and Trm1p play a critical role in the transcriptional regulation of genes of MUT pathway of K. phaffii.
| Item Type: | Journal Article |
|---|---|
| Publication: | Frontiers in Microbiology |
| Publisher: | Frontiers Media S.A. |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | Gcn5; histone acetyltransferase; Komagataella phaffii; methanol utilization pathway; MXR1; RNA-Seq; SNF1; Trm1 |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 08 Oct 2022 04:11 |
| Last Modified: | 08 Oct 2022 04:11 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77291 |
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