Kumar, SD and Kar, D and Akhtar, MN and Willard, B and Roy, D and Hussain, T and Rajyaguru, PI and Eswarappa, SM (2022) Evidence for low-level translation in human erythrocytes. In: Molecular biology of the cell, 33 (12). br21.
Full text not available from this repository.Abstract
It is generally believed that human mature erythrocytes do not possess functional ribosomes and therefore cannot synthesize proteins. However, the absence of translation is not consistent with the long lifespan of mature erythrocytes. They stay viable and functional for about 115 d in the circulatory system. Here, using a highly pure preparation of human mature erythrocytes, we demonstrate the presence of translation by polysome profiling, 35Smethionine labeling, and RiboPuromycylation. 35Smethionine labeling revealed that the translation in mature erythrocytes is about 10% of that observed in reticulocytes. We could observe polysomes by transmission electron microscopy in these cells. RNA-seq and quantitative real-time PCR performed on polysome fractions of these cells revealed that HBA (α-globin) and HBB (β-globin) transcripts are translated. Using a luciferase-based reporter assay and mutational studies, we show that the sequence of the 5' untranslated region is crucial for the translation of these transcripts. Furthermore, mature erythrocytes showed reduced expression of globin proteins (α- and β-) when treated with translation inhibitors. Overall, we provide multiple lines of evidence for translation of globin mRNAs in human mature erythrocytes.
| Item Type: | Journal Article |
|---|---|
| Publication: | Molecular biology of the cell |
| Publisher: | NLM (Medline) |
| Additional Information: | The copyright for this article belongs to NLM (Medline). |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 12 Oct 2022 04:46 |
| Last Modified: | 12 Oct 2022 04:46 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77283 |
Actions (login required)
 |
View Item |
