Aptamer-DNA Origami-Functionalized Solid-State Nanopores for Single-Molecule Sensing of G-Quadruplex Formation

Pal, S and Naik, A and Rao, A and Chakraborty, B and Varma, MM (2022) Aptamer-DNA Origami-Functionalized Solid-State Nanopores for Single-Molecule Sensing of G-Quadruplex Formation. In: ACS Applied Nano Materials, 5 (7). pp. 8804-8810.

PDF ACS_app_nan_mat_5-7_8804-8810_2022.pdf - Published Version Restricted to Registered users only Download (5MB) | Request a copy

Abstract

We describe the single-molecule sensing of G-quadruplex (G-q) formation in target-bound aptamers using solid-state nanopores. In order to do this, we functionalize the solid-state nanopore using DNA origami sheets incorporated with the aptamer sequence. This provides a modular configuration for nanopore-based single-molecule sensors where changing the aptamer sequence can produce the required origami functionalization of the nanopore. Furthermore, the DNA origami sheet also contains a pore which can modulate the translocation dynamics of the target molecule through the nanopore. Using this system, we show that we can distinguish the formation of G-q formation in the well-studied thrombin aptamer. © 2022 American Chemical Society.

Item Type:	Journal Article
Publication:	ACS Applied Nano Materials
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to the American Chemical Society.
Keywords:	Enzymes; Nanopores, Aptamer sequences; Aptamers; DNA origami; Functionalized; G-quadruplexes; Hybrid nanopore; Quadruplex formation; Single molecule; Solid-state nanopore; Thrombin, DNA
Department/Centre:	Division of Mechanical Sciences > Chemical Engineering Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	06 Oct 2022 10:08
Last Modified:	06 Oct 2022 10:08
URI:	https://eprints.iisc.ac.in/id/eprint/77203

Actions (login required)

View Item