Dopamine fluorescent sensor based on green synthesized copper oxide nanoparticles and tyrosinase

Pavithra, N and Johri, S and Ramamurthy, PC (2022) Dopamine fluorescent sensor based on green synthesized copper oxide nanoparticles and tyrosinase. In: 2022 IEEE International Conference on Flexible and Printable Sensors and Systems, FLEPS 2022, 10 July 2022 13 July 2022, Vienna.

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Abstract

Dopamine is a catecholamine that is an essential neurotransmitter in the human body. Thus, even a minute variation in its concentration and metabolism leads to severe neurological damage or diseases such as Parkinson's, Alzheimer's, Schizophrenia, and many more. It could be prevented at some scale by early detection. Nanoparticles have a remarkable ability to be used in sensors for detection purposes. This study synthesized copper oxide nanoparticles by green synthesis (P-Cu2O NPs) with Artemisia absinthium leaf extract for dopamine detection, accompanied by the enzyme tyrosinase used as a sensing material. The structural characterization of the nanoparticles and its interaction with dopamine via fluorescence spectroscopy was carried out to determine the sensitivity. The combination (P-Cu2O + tyrosinase) showed selective response to dopamine and did not show any response to interfering analyte like ascorbic acid, cysteine, and tyrosine. The limit of detection (LOD) of dopamine is observed to be 5 µM. This study indicates that P-Cu2O + tyrosinase can be a fluorescent probe in detecting dopamine.

Item Type:	Conference Paper
Publication:	FLEPS 2022 - IEEE International Conference on Flexible and Printable Sensors and Systems, Proceedings
Publisher:	Institute of Electrical and Electronics Engineers Inc.
Additional Information:	The copyright for this article belongs to Institute of Electrical and Electronics Engineers Inc.
Keywords:	Amines; Amino acids; Ascorbic acid; Copper oxides; Fluorescence; Fluorescence spectroscopy; MEMS; Neurophysiology; Synthesis (chemical), Alzheimer; Artemisia absinthium; Copper oxide nanoparticles; Dopamine; Fluorescent sensors; Green synthesis; Human bodies; Leaf extracts; Synthesised; Tyrosinase, Nanoparticles
Department/Centre:	Division of Interdisciplinary Sciences > Interdisciplinary Centre for Water Research
Date Deposited:	05 Oct 2022 09:30
Last Modified:	05 Oct 2022 09:30
URI:	https://eprints.iisc.ac.in/id/eprint/77059

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