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TEM based applications in solid state nanopores: From fabrication to liquid in-situ bio-imaging

P, MS and Simran, S and Nukala, P and Varma, MM (2022) TEM based applications in solid state nanopores: From fabrication to liquid in-situ bio-imaging. In: Micron, 162 .

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Official URL: https://doi.org/10.1016/j.micron.2022.103347


Nanopore-based techniques are widely used owing to their diverse applications such as DNA sequencing, ion detection, gas filtration, protein sequencing, and numerous other applications. Although commercialized sequencing methods are based on biological nanopores, solid-state nanopore technology is emerging due to its several advantages over biological nanopores, such as its tunable size, chemical and mechanical stability, and possibilities for easy integration with measurement electronics. The unavailability of rapid, low-cost, easy solid-state nanopore fabrication methods with industrial scalability is one of the current bottlenecks in this domain. Among all nanopore fabrication techniques, the Transmission electron microscope (TEM) based fabrication method is frequently used in research labs due to its capability of drilling and tuning nanopores with high accuracy. Given that there are no other methods capable of imaging and fabricating nanopores simultaneously, it is important to discuss the related methods and protocols of TEM. This review focuses on the various aspects of nanopore technology using TEM, from pore fabrication to imaging. Hybrid nanopores are also emerging, which combine the benefits of biological and solid-state nanopores. These can be formed by integrating DNA origami with solid-state nanopores. Creating and imaging DNA origami structures also presents several challenges. We also review DNA origami imaging using conventional TEM. We hope that this review will provide a one-stop reference to TEM applications on solid-state nanopores from fabrication to bioimaging and boost further research in this area.

Item Type: Journal Article
Publication: Micron
Publisher: Elsevier Ltd
Additional Information: The copyright for this article belongs to Elsevier Ltd.
Keywords: Chemical stability; Costs; DNA; DNA sequences; Gene encoding; High resolution transmission electron microscopy; Mechanical stability; Nanopores, Bio-imaging; Diverse applications; DNA origami; DNA Sequencing; Fabrication method; Ion detection; Liquid transmission electron microscope; Solid state nanopore fabrication; Solid-state nanopore; Transmission electron, Fabrication
Department/Centre: Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited: 05 Oct 2022 04:50
Last Modified: 05 Oct 2022 04:50
URI: https://eprints.iisc.ac.in/id/eprint/77016

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