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Visualizing the anti-cancer effects of chrysin nanoparticles by flow cytometry, microscopy and Fourier transform infrared spectroscopy

Kamat, S and Kumari, M and Jayabaskaran, C (2022) Visualizing the anti-cancer effects of chrysin nanoparticles by flow cytometry, microscopy and Fourier transform infrared spectroscopy. In: Colloidal Nanoparticles for Biomedical Applications XVII 2022, 20 - 24 February 2022, Virtual, Online.

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Official URL: https://doi.org/10.1117/12.2621529


Nano formulations of anti-cancer natural products have revolutionized anti-cancer therapy and its challenges such as short half-life, low therapeutic index, non-specificity, efficiency, and bioavailability. Chrysin (Chr), a dihydroxyflavone isolated from a marine fungus Chaetomium globosum, is a known anti-cancer natural product. However, its free use in clinical scenarios is disadvantaged due to poor water solubility, rapid metabolism, and non-specific toxicity. In this study, Chr-nanoparticles (NPs) were prepared by the emulsion-diffusion evaporation technique. The optimized formulation of Chr-NPs was characterized for zeta size, potential, polydispersity index, transmission electron microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FTIR). Chr-NPs had an average zeta size of 217.93 ± 3.45 nm, and a zeta potential of -15.63 ± 3.9 mV. As seen under the TEM, Chr-NPs measured an average size of 98.55 ± 4.01 nm. The NPs demonstrated an initial burst followed by a sustained release behaviour. The anti-cancer effects of Chr-NPs were investigated in human cervical cancer cells (HeLa) and human embryonic kidney (HEK) cells. MTT and propidium iodide live/dead flow cytometry assays demonstrated an improved IC50 value over the free drug treatment. An interplay of apoptosis and autophagy cell death mechanisms in Chr-NP treated HeLa cells was observed by Acridine Orange and MDC staining, visualized by confocal microscopy. Chr-NPs induced structural changes in the HeLa cell surface proteins and lipids were studied by FTIR spectroscopy. Natural products are the largest source of drug discovery; this study can pave the way to improve their chemistry sustainably and explore the cell death mechanisms. © 2022 SPIE

Item Type: Conference Paper
Publication: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Publisher: SPIE
Additional Information: The copyright for this article belongs to the SPIE.
Keywords: Biochemistry; Cancer cells; Cell membranes; Diseases; Flavonoids; Flow cytometry; Fourier transform infrared spectroscopy; High resolution transmission electron microscopy; Lanthanum compounds; Nanoparticles; Targeted drug delivery; Zeta potential, Anti-cancer therapies; Anticancer; Autophagy; Chrysin; Fourier transform infrared spectroscopy spectroscopy; Half lives; HeLa cell; Natural products; Spectroscopy:spectroscopy; Therapeutic index, Cell death
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 03 Oct 2022 05:27
Last Modified: 03 Oct 2022 05:27
URI: https://eprints.iisc.ac.in/id/eprint/76859

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