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Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton�s Jelly Mesenchymal Stem Cells

Singh, SK and Singh, A and Kumar, V and Gupta, J and Umrao, S and Kumar, M and Sarma, DK and Leja, M and Bhandari, MP and Verma, V (2022) Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton�s Jelly Mesenchymal Stem Cells. In: International Journal of Molecular Sciences, 23 (10).

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Official URL: https://doi.org/10.3390/ijms23105816


Mesenchymal stem cell (MSC)-based therapy and tissue repair necessitate the use of an ideal clinical biomaterial capable of increasing cell proliferation and differentiation. Recently, MXenes 2D nanomaterials have shown remarkable potential for improving the functional properties of MSCs. In the present study, we elucidated the potential of Ti2CTx MXene as a biomaterial through its primary biological response to human Wharton�s Jelly MSCs (hWJ-MSCs). A Ti2CTx nanosheet was synthesized and thoroughly characterized using various microscopic and spectro-scopic tools. Our findings suggest that Ti2CTx MXene nanosheet exposure does not alter the morphology of the hWJ-MSCs; however, it causes a dose-dependent (10�200 µg/mL) increase in cell proliferation, and upon using it with conditional media, it also enhanced its tri-lineage differentiation potential, which is a novel finding of our study. A two-fold increase in cell viability was also noticed at the highest tested dose of the nanosheet. The treated hWJ-MSCs showed no sign of cellular stress or toxicity. Taken together, these findings suggest that the Ti2CTx MXene nanosheet is capable of augmenting the proliferation and differentiation potential of the cells. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Item Type: Journal Article
Publication: International Journal of Molecular Sciences
Publisher: MDPI
Additional Information: The copyright for this article belongs to the Authors.
Keywords: biomaterial; immunologic factor, cell differentiation; human; mesenchymal stem cell; physiology; Wharton jelly, Biocompatible Materials; Cell Differentiation; Humans; Immunologic Factors; Mesenchymal Stem Cells; Wharton Jelly
Department/Centre: Division of Physical & Mathematical Sciences > Physics
Date Deposited: 29 Sep 2022 06:15
Last Modified: 29 Sep 2022 06:15
URI: https://eprints.iisc.ac.in/id/eprint/76820

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