Soni, S and Toley, BJ (2021) PAPER-BASED PURIFICATION AND CONCENTRATION OF M. TUBERCULOSIS GENOMIC DNA USING ISOTACHOPHORESIS. In: 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, 10 October 2021through 14 October 2021, Palm Springs, Virtual, pp. 687-688.
Full text not available from this repository. (Request a copy)Abstract
In recent years, much work and effort have been put into developing affordable and simple POC-nucleic acid amplification tests (NAATs) for diagnosing infectious diseases like tuberculosis. While the primary focus has been on nucleic acid (NA) amplification and detection strategies, many POC-NAAT assays still rely on complex sample preparation methods. To overcome this limitation, we demonstrate a POC-compatible paper-based device capable of purifying and concentrating Mycobacterium tuberculosis genomic DNA (gDNA) from viscous mock-sputum using isotachophoresis. This is the first demonstration of sputum DNA preparation using paper-based ITP. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
| Item Type: | Conference Paper |
|---|---|
| Publication: | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher: | Chemical and Biological Microsystems Society |
| Additional Information: | The copyright for this article belongs to Chemical and Biological Microsystems Society. |
| Keywords: | isotachophoresis (ITP); NAAT; Paperfluidics; point-of-care (POC) diagnostics; sample preparation |
| Department/Centre: | Division of Mechanical Sciences > Chemical Engineering |
| Date Deposited: | 03 Oct 2022 05:23 |
| Last Modified: | 03 Oct 2022 05:23 |
| URI: | https://eprints.iisc.ac.in/id/eprint/76689 |
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