Agarwal, P and Toley, BJ (2021) UNREACTED PCR PRIMERS INHIBIT SIGNAL IN A NUCLEIC ACID LATERAL FLOW ASSAY: A TRANSPORT REACTION MODEL ELUCIDATES. In: 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, 10 - 14 October 2021, Palm Springs, Virtual, pp. 1101-1102.
Full text not available from this repository.Abstract
This study presents the first transport-reaction model of a nucleic acid lateral flow assay (NALFA). We prove that in NALFA, in addition to the hook effect, the presence of unreacted primers in the polymerase chain reaction (PCR) product is also responsible for the reduction in test line intensity; we demonstrate this both experimentally and using the transport-reaction model. These results elucidate that the performance of a NALFA is tightly coupled to the design and conduct of the PCR reaction. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
| Item Type: | Conference Paper |
|---|---|
| Publication: | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher: | Chemical and Biological Microsystems Society |
| Additional Information: | The copyright for this article belongs to Chemical and Biological Microsystems Society. |
| Keywords: | Hook effect; Nucleic acid lateral flow assay; Point-of-care diagnostics; Transport reaction model |
| Department/Centre: | Division of Mechanical Sciences > Chemical Engineering |
| Date Deposited: | 03 Oct 2022 05:04 |
| Last Modified: | 06 Oct 2022 06:04 |
| URI: | https://eprints.iisc.ac.in/id/eprint/76688 |
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