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Single-cell analysis reveals X upregulation is not global in pre-gastrulation embryos

Naik, HC and Hari, K and Chandel, D and Jolly, MK and Gayen, S (2022) Single-cell analysis reveals X upregulation is not global in pre-gastrulation embryos. In: iScience, 25 (6).

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Official URL: https://doi.org/10.1016/j.isci.2022.104465


In mammals, transcriptional inactivation of one X chromosome in female compensates for the dosage of X-linked gene expression between the sexes. Additionally, it is believed that the upregulation of active X chromosome in male and female balances the dosage of X-linked gene expression relative to autosomal genes, as proposed by Ohno. However, the existence of X chromosome upregulation (XCU) remains controversial. Here, we have profiled gene-wise dynamics of XCU in pre-gastrulation mouse embryos at single-cell level and found that XCU is dynamically linked with X chromosome inactivation (XCI); however, XCU is not global like XCI. Moreover, we show that upregulated genes are enriched with activating marks and have enhanced burst frequency. Finally, our In-silico model predicts that recruitment probabilities of activating factors and a surge of these factors upon X-inactivation trigger XCU. Altogether, our study provides significant insight into the gene-wise dynamics and mechanistic basis of XCU during early development and extends support for Ohno's hypothesis.

Item Type: Journal Article
Publication: iScience
Publisher: Elsevier Inc.
Additional Information: The copyright for this article belongs to the Authors.
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Date Deposited: 21 Sep 2022 06:07
Last Modified: 21 Sep 2022 06:07
URI: https://eprints.iisc.ac.in/id/eprint/76617

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