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Casein-Coated Molybdenum Disulfide Nanosheets Augment the Bioactivity of Alginate Microspheres for Orthopedic Applications

Appana Dalavi, P and Prabhu, A and Sajida, M and Chatterjee, K and Venkatesan, J (2022) Casein-Coated Molybdenum Disulfide Nanosheets Augment the Bioactivity of Alginate Microspheres for Orthopedic Applications. In: ACS Omega, 7 (30). pp. 26092-26106.

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Official URL: https://doi.org/10.1021/acsomega.2c00995


Defects and disorders of the bone due to disease, trauma, or abnormalities substantially affect a person's life quality. Research in bone tissue engineering is motivated to address these clinical needs. The present study demonstrates casein-mediated liquid exfoliation of molybdenum disulfide (MoS2) and its coupling with alginate to create microspheres to engineer bone graft substitutes. Casein-exfoliated nano-MoS2was chemically characterized using different analytical techniques. The UV-visible spectrum of nano-MoS2-2 displayed strong absorption peaks at 610 and 668 nm. In addition, the XPS spectra confirmed the presence of the molybdenum (Mo, 3d), sulfur (S, 2p), carbon (C, 1s), oxygen (O, 1s), and nitrogen (N, 1s) elements. The exfoliated MoS2nanosheets were biocompatible with the MG-63, MC3T3-E1, and C2C12 cells at 250 μg/mL concentration. Further, microspheres were created using alginate, and they were characterized physiochemically and biologically. Stereomicroscopic images showed that the microspheres were spherical with an average diameter of 1 ± 0.2 mm. The dispersion of MoS2in the alginate matrix was uniform. The alginate-MoS2microspheres promoted apatite formation in the SBF (simulated body fluid) solution. Moreover, the alginate-MoS2was biocompatible with MG-63 cells and promoted cell proliferation. Higher alkaline phosphatase activity and mineralization were observed on the alginate-MoS2with the MG-63 cells. Hence, the developed alginate-MoS2microsphere could be a potential candidate for a bone graft substitute.

Item Type: Journal Article
Publication: ACS Omega
Publisher: American Chemical Society
Additional Information: The copyright for this article belongs to the Author(s).
Department/Centre: Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited: 17 Sep 2022 05:23
Last Modified: 17 Sep 2022 05:23
URI: https://eprints.iisc.ac.in/id/eprint/76589

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