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Gap junction β-2 expression is negatively associated with the estrogen receptor status in breast cancer tissues and is a regulator of breast tumorigenesis

Shettar, A and Damineni, S and Mukherjee, G and Kondaiah, P (2018) Gap junction β-2 expression is negatively associated with the estrogen receptor status in breast cancer tissues and is a regulator of breast tumorigenesis. In: Oncology Reports, 40 (6). pp. 3645-3653.

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Official URL: https://doi.org/10.3892/or.2018.6764

Abstract

Gap junction β-2 gene (GJB2, also known as connexin 26) is a member of the connexin family which forms gap junction channels. Many connexin genes have been considered to be tumor suppressor genes. However, the overexpression of GJB2 has been found to be associated with a poor prognosis in several human cancers. In our previous microarray study, we revealed the overexpression of GJB2 in breast cancer tissues. Hence, in this study, we investigated the expression of GJB2 in human breast cancer and its role in breast cancer cell proliferation and migration. The RT-qPCR results revealed the upregulation of the GJB2 gene in invasive ductal carcinoma (P<0.001) of the breast. Immunohistochemical analysis revealed an intense cytoplasmic and membrane staining. We observed that the staining for GJB2 was more intense in the majority of the estrogen receptor (ER).negative breast cancer tissues compared to the normal breast tissues (P<0.0001). By contrast, the majority of the ER-positive breast cancer samples exhibited weak to moderate staining; however, this difference was not statistically significant compared to the normal tisues. The knockdown of GJB2 in human breast cancer cell lines using shRNA led to a significant decrease in the proliferative ability and an increase in the migratory ability of breast cancer cells. In addition, the knockdown of GJB-2 led to a significant reduction in tumor volume and proliferation (as demonstrated by MIB-1 staining) in orthotopic xenografts in immunocompromised mice. On the whole, the findings of this study indicate that GJB2 may be an important regulator of breast tumorigenesis.

Item Type: Journal Article
Publication: Oncology Reports
Publisher: Spandidos Publications
Additional Information: The copyright for this article belongs to the Authors.
Keywords: connexin 26; estrogen receptor; short hairpin RNA; DFNA3 protein, human; estrogen receptor; gap junction protein, adult; aged; animal experiment; animal model; animal tissue; Article; breast cancer cell line; breast carcinoma; breast tissue; cancer size; cancer tissue; carcinogenesis; cell membrane; cell migration; cell proliferation; controlled study; cytoplasm; estrogen receptor negative breast cancer; estrogen receptor positive breast cancer; female; gap junction; gene expression; gene expression regulation; gene function; gene knockdown; GJB2 gene; human; human cell; human tissue; immunohistochemistry; major clinical study; middle aged; nonhuman; priority journal; regulatory mechanism; reverse transcription polymerase chain reaction; tumor xenograft; upregulation; animal; breast; breast tumor; carcinogenesis; cell motion; genetics; metabolism; mouse; nude mouse; Paget nipple disease; pathology; tumor cell line, Animals; Breast; Breast Neoplasms; Carcinogenesis; Carcinoma, Ductal, Breast; Cell Line, Tumor; Cell Movement; Cell Proliferation; Connexins; Female; Gene Knockdown Techniques; Humans; Mice; Mice, Nude; Receptors, Estrogen; Up-Regulation
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 23 Aug 2022 09:04
Last Modified: 23 Aug 2022 09:05
URI: https://eprints.iisc.ac.in/id/eprint/76196

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