Evaluation of Antithrombotic Activities of Solanum xanthocarpum and Tinospora cordifolia

Lugun, O and Bhoi, S and Kujur, P and Kumar, D and Surin, WR (2018) Evaluation of Antithrombotic Activities of Solanum xanthocarpum and Tinospora cordifolia. In: Pharmacognosy Research, 10 (1). pp. 98-103.

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Abstract

Background: Solanum xanthocarpum and Tinospora cordifolia have been reported to exhibit anti-inflammatory, antiarthritic, antioxidant, antiallergic, and hepatoprotective activities. The origins of many of the currently available antithrombotic treatments are from natural products and natural sources. Objective: To investigate the antithrombotic activities of methanolic leaf extracts of S. xanthocarpum(SXME) and T. cordifolia(TCME). Materials and Methods: Antithrombotic activities were assessed by thrombin inhibition assay, thrombin generation assay, platelet adhesion assay on collagen-coated surface, and platelet PAC1-FITC binding by flow cytometry. Results: SXME significantly inhibited thrombin activity at 5-20 mg/ml concentrations, whereas TCME inhibited thrombin activity at 500 μg/ml-5 mg/ml concentrations. Further, SXME inhibited thrombin generation at 2-20 mg/ml concentrations, whereas TCME exhibited significant inhibition at 200 μg/ml, suggesting that TCME has higher efficacy as compared to SXME. Moreover, SXME did not inhibit platelet adhesion on collagen-coated surface, whereas TCME inhibited platelet adhesion on collagen-coated surface at 5 mg/ml. Indomethacin showed significant inhibition in platelet adhesion at 300 μM. Further, SXME inhibited thrombin-induced platelet activation (PAC1-FITC binding) significantly at 1 mg/ml by about 80, whereas TCME inhibited thrombin-induced platelet activation (PAC1-FITC binding) by about 40 at 1 mg/ml. Conclusion: These results strongly suggested that SXME and TCME possess antithrombotic activities. However, further studies are essential to find out the active constituent responsible for antithrombotic effect.

Item Type:	Journal Article
Publication:	Pharmacognosy Research
Publisher:	Medknow Publications
Additional Information:	The copyright for this article belongs to Medknow Publications.
Keywords:	anticoagulant agent; fluorescein isothiocyanate; methanol; plant extract; Solanum xanthocarpum extract; Tinospora cordifolia extract; unclassified drug, animal experiment; antithrombotic activity; Article; controlled study; drug activity; drug determination; flow cytometry; in vitro study; male; nonhuman; plant leaf; platelet adhesion assay; rat; Solanum virginianum; thrombin generation assay; thrombin inhibition assay; thrombocyte activation; thrombocyte adhesion; Tinospora cordifolia
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	22 Aug 2022 09:14
Last Modified:	22 Aug 2022 09:14
URI:	https://eprints.iisc.ac.in/id/eprint/76119

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