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Generation of inducible transgenic lines of arabidopsis transcription factors regulated by microRNAs

Aggarwal, P and Challa, KR and Rath, M and Sunkara, P and Nath, U (2018) Generation of inducible transgenic lines of arabidopsis transcription factors regulated by microRNAs. [Book Chapter]

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Official URL: https://doi.org/10.1007/978-1-4939-8657-6_4


Transcription factors play key regulatory roles in all the life processes across kingdoms. In plants, the genome of a typical model species such as Arabidopsis thaliana encodes over 1500 transcription factors that regulate the expression dynamics of all the genes in time and space. Therefore, studying their function by analyzing the loss and gain-of-function lines is of prime importance in basic plant biology and its agricultural application. However, the current approach of knocking out genes often causes embryonic lethal phenotype, while inactivating one or two members of a redundant gene family yields little phenotypic changes, thereby making the functional analysis a technically challenging task. In such cases, inducible knock-down or overexpression of transcription factors appears to be a more effective approach. Restricting the transcription factors in the cytoplasm by fusing them with animal glucocorticoid/estrogen receptors (GR/ER) and then re-localizing them to the nucleus by external application of animal hormone analogues has been a useful method of gene function analysis in the model plants. In this chapter, we describe the recent advancements in the GR and ER expression systems and their use in analyzing the function of transcription factors in Arabidopsis.

Item Type: Book Chapter
Publication: Methods in Molecular Biology
Series.: Methods in Molecular Biology
Publisher: Humana Press Inc.
Additional Information: The copyright for this article belongs to the Springer Science+Business Media, LLC, part of Springer Nature.
Keywords: actin; Arabidopsis protein; complementary DNA; dexamethasone; estradiol; estrogen receptor; glucocorticoid receptor; microRNA; plant DNA; plant RNA; transcription factor; tubulin; ubiquitin; dexamethasone; glucocorticoid receptor; microRNA; transcription factor, Arabidopsis thaliana; DNA synthesis; gain of function mutation; gene control; gene function; gene targeting; loss of function mutation; mutant; nonhuman; plant gene; plant growth; plant seed; promoter region; rat; regulatory mechanism; RNA interference; RNA isolation; transgenic plant; Arabidopsis; drug effect; gene expression regulation; genetics; metabolism; molecular biology; procedures; real time polymerase chain reaction; reproducibility; transgenic plant, Arabidopsis; Dexamethasone; Gene Expression Regulation, Plant; MicroRNAs; Molecular Biology; Plants, Genetically Modified; Real-Time Polymerase Chain Reaction; Receptors, Glucocorticoid; Reproducibility of Results; Transcription Factors
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 30 Aug 2022 04:57
Last Modified: 30 Aug 2022 04:57
URI: https://eprints.iisc.ac.in/id/eprint/76035

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