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Nanoscale regulation of Ca2+ dependent phase transitions and real-time dynamics of SAP97/hDLG

Rajeev, P and Singh, N and Kechkar, A and Butler, C and Ramanan, N and Sibarita, JB and Jose, M and Nair, D (2022) Nanoscale regulation of Ca2+ dependent phase transitions and real-time dynamics of SAP97/hDLG. In: Nature Communications, 13 (1).

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Official URL: https://doi.org/10.1038/s41467-022-31912-1


Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. We confirm that the molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Furthermore, the nanoscale molecular signatures of these signalling islands and phase transitions are altered in response to changes in cytosolic Ca2+. Additionally, exchange kinetics of alternatively spliced isoforms of the intrinsically disordered region in SAP97/hDLG C-terminus shows differential sensitivities to Ca2+ bound Calmodulin, affirming that the molecular signatures of local phase transitions of SAP97/hDLG depends on their nanoscale heterogeneity and compositionality of isoforms.

Item Type: Journal Article
Publication: Nature Communications
Publisher: Nature Research
Additional Information: The copyright for this article belongs to the Authors.
Keywords: heterogeneity; phase transition; real time; vertebrate
Department/Centre: Division of Biological Sciences > Centre for Neuroscience
Date Deposited: 10 Aug 2022 05:26
Last Modified: 10 Aug 2022 05:26
URI: https://eprints.iisc.ac.in/id/eprint/75760

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