Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3

Ayyub, SA and Aswathy, SL and Dobriyal, D and Aluri, S and Spremulli, LL and Varshney, U (2018) Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3. In: Mitochondrion, 39 . pp. 1-8.

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Abstract

Initiation factor 3 (IF3) is a conserved translation factor. Mutations in mitochondrial IF3 (IF3mt) have been implicated in disease pathology. Escherichia coli infCΔ55, compromised for IF3 activity, has provided an excellent heterologous system for IF3mt structure-function analysis. IF3mt allowed promiscuous initiation from AUA, AUU and ACG codons but avoided initiation with initiator tRNAs lacking the conserved 3GC pairs in their anticodon stems. Expression of IF3mt N-terminal domain, or IF3mt devoid of its typical N-, and C-terminal extensions improved fidelity of initiation in E. coli. The observations suggest that the IF3mt terminal extensions relax the fidelity of translational initiation in mitochondria.

Item Type:	Journal Article
Publication:	Mitochondrion
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to the Elsevier B.V.
Keywords:	adenine; cytosine; guanine; initiation factor 3; mitochondrial protein; transfer RNA; uracil; initiation factor 3; mitochondrial protein; recombinant protein; transfer RNA, amino terminal sequence; anticodon; Article; carboxy terminal sequence; codon; controlled study; Escherichia coli; genetic conservation; mammal cell; mitochondrion; nonhuman; priority journal; protein domain; protein expression; protein function; RNA transport; translation initiation; enzymology; genetics; human; metabolism; start codon, Codon, Initiator; Escherichia coli; Humans; Mitochondrial Proteins; Peptide Chain Initiation, Translational; Prokaryotic Initiation Factor-3; Recombinant Proteins; RNA, Transfer
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	10 Aug 2022 05:09
Last Modified:	10 Aug 2022 05:09
URI:	https://eprints.iisc.ac.in/id/eprint/75652

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